Earticle

초록

영어

A variety of toxic bloom-forming algae damaged marine ecosystems in the ocean. Recently, the viruses infecting the harmful algae were isolated and characterized. It was found that the viruses control the population dynamics by terminating the harmful algae. Previously, the HcRNAV strains infecting Heterocapsa circularisquama that causes HAB (Harmful algal blooming) were isolated and their intraspecies infectivity was discovered. The genome sequencing of the viruses identified a capsid gene and it was speculated that the variable regions in the capsid surfaces determined the specificities. To utilize the capsid as tool of HAB control, the expression and purification of the proteins were attempted in this study. To express the capsid protein from one of the HcRNAV strains, HcRNAV109, the gene encoding the protein was synthesized for codon optimization. The gene was cloned into pTXB1 expression vector containing intein tag gene and its expression was induced by IPTG in E.coli BL21(DE3). The recombinant protein was purified with chitin affinity chromatography column and its formation into the viral like protein (VLP) was confirmed by transmission electron microscopy (TEM). To ensure attachment of the recombinant capsid into the algae with host specificity, it was labeled with fluorescein isothiocyanate (FITC) during a chemical dissociation and reassociation process. The following infection experiment showed that the capsid of HcRNAV109 exhibited specific affinity to H. circularisquama strain HY9423.

키워드

저자

• Hye Gyung OH [ Department of Environmental Engineering, BK21 Team for Biohydrogen Production & Pioneer Research Center for Controlling of Harmful Algal Bloom, Chosun University, Gwangju 501-759. ]
• Won Duck KIM [ Department of Environmental Engineering, BK21 Team for Biohydrogen Production & Pioneer Research Center for Controlling of Harmful Algal Bloom, Chosun University, Gwangju 501-759. ]
• Seo Sun IL [ Department of Environmental Engineering, BK21 Team for Biohydrogen Production & Pioneer Research Center for Controlling of Harmful Algal Bloom, Chosun University, Gwangju 501-759. ]
• Si Wouk KIM [ Department of Environmental Engineering, BK21 Team for Biohydrogen Production & Pioneer Research Center for Controlling of Harmful Algal Bloom, Chosun University, Gwangju 501-759. ]

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발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  한국생물공학회 학술대회

• 간기

  반년간

• 수록기간

  1985~2013

• 십진분류

  KDC 476 DDC 576