To obtain higher biomass productivity from Chlorella sp. culture, heterotrophic cultivations which supplements organic carbon sources into the autotrophic cultures in a photobioreactor was performed. In our study, heterotrophic media was prepared by adding various carbohydrates as well as yeast extract. The biomass of Chlorella sp. obtained from the autotrophic cultivation was 1.36 g/L. However, when each of 0.01 M glucose, xylose or galactose was added, the biomass increased to 1.76, 1.42, 1.47 g/L, respectively. In addition, rhamnose did not affect the biomass production and fructose and sucrose reversely inhibited the growth of Chlorella sp. 0.8 g/L yeast extract stimulated the highest biomass production (7.78 g/L), which is five-fold higher than that from autotrophic cultivation. It was found that the addition of yeast extract increased significantly the growth rate of microalga, namely, the highest biomass was from 6 day incubation while it took 12 days in cases of the autotrophic or heterotrophic growth with carbohydrates.
한국생물공학회 [The Korean Society for Biotechnology and Bioengineering]
설립연도
1984
분야
공학>생물공학
소개
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