Earticle

초록

영어

Virus-like particles (VLPs) have received considerable attention for vaccine, drug delivery, gene therapy and material science applications. Recombinantly expressed VLPs of HcRNAV, a single-stranded RNA virus specifically infecting the dinoflagellate, Heterocapsa circularisquama, were described recently as a new carrier system to suppress harmful algal blooms. HcRNAV strains were divided into two types (HcRNAV34 and HcRNAV109) by complementary strain-specific infectivity. In the present study, recombinant HcRNAV34 VLPs were expressed in E. coli and purified by chitin-binding affinity column chromatography. A novel organic synthesized algicidal substance, thiazolidinedione (TD49), was packaged into HcRNAV34 VLPs by dissociation and reassociation processes and its encapsulation was detected by HPLC analysis. HcRNAV34 VLPs encapsulating TD49 specifically attached and destroyed the cells of H. circularisquam HU9433-P, which is the host of HcRNAV34 virus, but not the cells of HY9423, which is the host of HcRNAV109 virus.

키워드

저자

• Sun Yi JU [ Dept. of Environmental Engineering, BK 21 Team for Biohydrogen Production, Cosun University, Gwnagju, 501-759. ]
• Pyoung Il KIM [ Department of Environmental Engineering, BK21 Team for Biohydrogen Production &Pioneer Research Center for Controlling of Harmful Algal Bloom, Chosun University, Gwangju 501-759. ]
• Jae Won RYU [ Pioneer Research Center for Controlling of Harmful Algal Bloom, Chosun University, Gwangju 501-759, Korea. ]
• Geun Ho GIM [ Engineering, BK21 Team for Biohydrogen Production &Pioneer Research Center for Controlling of Harmful Algal Bloom, Chosun University, Gwangju 501-759. ]
• Si Wouk KIM [ Engineering, BK21 Team for Biohydrogen Production &Pioneer Research Center for Controlling of Harmful Algal Bloom, Chosun University, Gwangju 501-759. ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  한국생물공학회 학술대회

• 간기

  반년간

• 수록기간

  1985~2013

• 십진분류

  KDC 476 DDC 576