Earticle

초록

영어

The cDNA of the glx1 gene encoding glyoxal oxidase 1 from Phanerochaete chrysosporium was isolated and expressed in a methylotrophic yeast Pichia pastoris. The recombinant Glx1 produced by P. pastoris showed a molecular mass of 70 kDa considerably larger than that of the native one (57 kDa) because of glycosylation of the former by P. pastoris and the enzyme activity reached the maximal value after 2 days of culture in the presence of methanol. The optimal temperature and pH of the recombinant Glx1 were at 30°C and 5, respectively. The optimal pH of decolorization of malachite green with a recombinant manganese peroxidase H4 (MnPH4) coupled with the recombinant Glx1 was 4.7. When Glx1 was added to the reaction mixture to produce H2O2 over than 7.0 nmol/min/mL, this coupling reaction showed higher decolorization rate than the reaction to which 0.1 mM of H2O2 was directly added. Within 90 minutes of incubation, this coupling reaction could rapidly decolorize malachite green up to 150 uM

키워드

저자

• Hyoun-Young KIM [ LED-Agribio Fusion Technology Research Center, Chonbuk National University, Iksan 570-752, Korea. ]
• Yu-Lim SON [ Division of Biotechnology, College of Environmental and Bioresource Sciences, Chonbuk National University, Iksan 570-752, Korea. ]
• Saravanakumar THIYAGARAJAN [ Division of Biotechnology, College of Environmental and Bioresource Sciences, Chonbuk National University, Iksan 570-752, Korea. ]
• Dae-Huyk KIM [ Institute for Molecular Biology and Genetics, Center for Fungal Pathogenesis, Chonbuk National University, Jeonju 561-756, Korea. ]
• Seung-Moon PARK [ Division of Biotechnology, College of Environmental and Bioresource Sciences, Chonbuk National University, Iksan 570-752, Korea. ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  한국생물공학회 학술대회

• 간기

  반년간

• 수록기간

  1985~2013

• 십진분류

  KDC 476 DDC 576