Nguyen Duc HUY, Ae-Yong MO, Hyoun-Young KIM, Seung-Moon PARK
언어
영어(ENG)
URL
https://www.earticle.net/Article/A174759
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원문정보
초록
영어
We cloned the cDNA of acetyl xylan esterase 2 (pcAXE2) gene from Phanerochate chrysosporium and expressed it into Pichia pastoris. The results indicated that the recombinant PcAXE2 (rPcAXE2) was efficiently produced and secreted, reaching at 938 Ul-1 after a 4 day induction. Molecular mass of the rPcAXE2 on SDS-PAGE was an approximately 63 kDa under hyperglycosylation. A biochemical analysis showed that rPcAXE2 has an optimum pH and temperature of 7.0 and 30-35oC, respectively, whereas specific activity, Km, and Vmax were 39.86 Umg-1, 92.2 μmol and 7.4 μmol min-1 ml-1, respectively. Moreover the rPcAXE2 also catalyzed the synthesis of peracetic acid (PAA) from ethyl acetate and hydrogen peroxide. The synthesized amount of PAA was 1.45 mM using 10 μg of rPcAXE2, which could be applied to the pretreatment of a cellulosic biomass in situ.
Nguyen Duc HUY [ Division of Biotechnology, College of Environmental and Bioresource sciences, Chonbuk National University, Iksan,570-752,Republic of Korea. ]
Ae-Yong MO [ Division of Biotechnology, College of Environmental and Bioresource Sciences, Chonbuk National University, Iksan 570-752, Korea. ]
Hyoun-Young KIM [ LED-Agribio Fusion Technology Research Center, Chonbuk National University, Iksan 570-752, Korea. ]
Seung-Moon PARK [ Division of Biotechnology, College of Environmental and Bioresource Sciences, Chonbuk National University, Iksan 570-752, Korea. ]
한국생물공학회 [The Korean Society for Biotechnology and Bioengineering]
설립연도
1984
분야
공학>생물공학
소개
이 법인은 생물 공학의 발전과 보급에 이바지하고, 회원 상호 간의 연구 협력과 친목을 도모함을 목적으로 한다
1. 생물공학 분야의 발전을 위한 연구 협력
2. 생물공학의 실용화를 촉진시키기 위한 산학 협동
3. 학술연구 발표회, 강연회, 연수회 등 학술활동의 개최
4. 국,영문 학술지,소식지,학술회의 Proceedings 및 학술도서의 발간
5. 생물공학 발전을 위한 정책 건의
6. 기타 국제 교류 등 본 학회의 목적 달성을 위한 제반 활동