Decolorization of D iazo, Triphenylmethane and Anthracene derivative Dyes by recombinant Manganese Peroxidase of Phanerochaete chrysosporium expressed in Pichia pastoris and its Effect of Decolorization with Redox Mediator
Saravanakumar THIYAGARAJAN, Hyoun-Young KIM, Seung-Moon PARK
언어
영어(ENG)
URL
https://www.earticle.net/Article/A174758
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원문정보
초록
영어
The cDNA of the glx1 gene encoding glyoxal oxidase 1 from Phanerochaete chrysosporium was isolated and expressed in a methylotrophic yeast Pichia pastoris. The recombinant Glx1 produced by P. pastoris showed a molecular mass of 70 kDa considerably larger than that of the native one (57 kDa) because of glycosylation of the former by P. pastoris and the enzyme activity reached the maximal value after 2 days of culture in the presence of methanol. The optimal temperature and pH of the recombinant Glx1 were at 30°C and 5, respectively. The optimal pH of decolorization of malachite green with a recombinant manganese peroxidase H4 (MnPH4) coupled with the recombinant Glx1 was 4.7. When G lx1 w as added to the reaction m ixture to produce H 2O2 over than 7.0 nmol/min/mL, this coupling reaction showed higher decolorization rate than the reaction to which 0.1 mM of H2O2 was directly added. Within 90 minutes of incubation, this coupling reaction could rapidly decolorize malachite green up to 150 uM.
Saravanakumar THIYAGARAJAN [ Division of Biotechnology, College of Environmental and Bioresource sciences, Chonbuk National University, Iksan,570-752,Republic of Korea. ]
Hyoun-Young KIM [ LED-Agribio Fusion Technology Research Center, Chonbuk National University, Iksan 570-752, Korea. ]
Seung-Moon PARK [ Division of Biotechnology, College of Environmental and Bioresource Sciences, Chonbuk National University, Iksan 570-752, Korea. ]
한국생물공학회 [The Korean Society for Biotechnology and Bioengineering]
설립연도
1984
분야
공학>생물공학
소개
이 법인은 생물 공학의 발전과 보급에 이바지하고, 회원 상호 간의 연구 협력과 친목을 도모함을 목적으로 한다
1. 생물공학 분야의 발전을 위한 연구 협력
2. 생물공학의 실용화를 촉진시키기 위한 산학 협동
3. 학술연구 발표회, 강연회, 연수회 등 학술활동의 개최
4. 국,영문 학술지,소식지,학술회의 Proceedings 및 학술도서의 발간
5. 생물공학 발전을 위한 정책 건의
6. 기타 국제 교류 등 본 학회의 목적 달성을 위한 제반 활동