Optimum condition of simultaneous saccharification and fermentation (SSF) of potato tuber mash for bioethanol production was examined. Central composite rotatable design(CCRD) and response surface methodology(RSM) were employed to find the optimal condition of SSF. Among four SSF factors such as saccharifying-enzyme(Spirizyme Fuel, Novo) dosage, ammonium sulfate content in medium, incubation temperature and yeast inoculum size, saccharifying-enzyme and incubation temperature were found to be important factors by using CCRD. Using RSM, the optimal saccharifying-enzyme dosage and incubation temperature were determined to be 1.45 AGU g-1 dry matter and 31.3℃, respectively. Using the optimal condition of SSF, 14.92% (v/v) ethanol was produced after 60 hr, and all the fermentable sugar was completely used up.
키워드
potatoethanolsimultaneous saccharification and fermentation
저자
You-ri JANG [ Dept. of Bioscience and Biotechnology, The University of Suwon, Gyeonggi-do 445-743, Korea. ]
Young-hoon LIM [ Dept. of Bioscience and Biotechnology, The University of Suwon, Gyeonggi-do 445-743, Korea. ]
Keun KIM [ Dept. of Bioscience and Biotechnology, The University of Suwon, Gyeonggi-do 445-743, Korea. ]
한국생물공학회 [The Korean Society for Biotechnology and Bioengineering]
설립연도
1984
분야
공학>생물공학
소개
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