Earticle

초록

영어

A microorganism producing carboxymethylcellulase (CMCase) was isolated from seawater, identified as Bacillus velezensis by analyses of 16S rDNA and partial sequences of the gyrA, and designated as B. velezensis A-68. The optimal conditions for production of CMCase by B. velezensis A-68 were established using response surface methodology (RSM). The optimal concentrations of rice hulls and yeast extract, and initial pH of the medium for cell growth were 60.2 g/L, 7.38 g/L, and 7.18, respectively, whereas those for production of CMCase were 50.0 g/L, 5.00 g/L, and 7.30. The analysis of variance (ANOVA) implied that the most significant factor for cell growth as well as production of CMCase was yeast extract. The optimal concentrations of K2HPO4, NaCl, MgSO4·7H2O, and (NH4)2SO4 in the medium for cell growth were 7.50, 1.00, 0.10, and 0.80 g/L, respectively, which were the same as those for production of CMCase. The ANOVA also indicated that highly significant (“probe>F” less than 0.0001) for cell growth was K2HPO4, whereas those for production of CMCase were K2HPO4, MgSO4·7H2O, and (NH4)2SO4. The optimal temperatures for cell growth and production of CMCase were 30 and 35℃, respectively. The maximal production of CMCase under optimized conditions was 83.8 U/mL, which was 3.3 times higher than that before optimization. In this study, rice hulls were developed as a substrate for production of CMCase and time for production of CMCase was reduced to 3 days using a newly isolated marine bacterium.

키워드

저자

• Wa GAO [ Department of Medical Bioscience of Graduate School of Dong-A University and Dong A University, Busan. ]
• Hyung-Woo Kim [ Department of Medical Bioscience of Graduate School of Dong-A University and Dong A University, Busan. ]
• Myung-Hwan Kim [ Department of Medical Bioscience of Graduate School of Dong-A University and Dong A University, Busan. ]
• Jin-Woo Lee [ BK21 Bio-Silver Program of Dong-A University, and Department of Biotechnology, Dong-A University, Busan 604-714, Korea ]

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간행물

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• 간행물명

  한국생물공학회 학술대회

• 간기

  반년간

• 수록기간

  1985~2013

• 십진분류

  KDC 476 DDC 576