Characterization and preliminary X-ray crystallographic analysis of an Ice-binding protein (FfIBP) from phychrophilic bacteria, Flavobacterium frigoris
Hackwon DO, Jun Hyuck LEE, Eunjung KIM, Sung Gu LEE, Hak Jun KIM
언어
영어(ENG)
URL
https://www.earticle.net/Article/A174711
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원문정보
초록
영어
The Ice binding protein (IBP) is a prerequisite material for organism to be allowed to live in a subzero environment. Ice growth in a cold environment is fatal for organism which is not just physically destruction of inner cell organelle but also chemical damage such an osmotic shock. IBP has been characterized property of which ability inhibit the ice growth by binding to specific ice plane. Flavobacterium frigoris isolated from Antarctic area produce an ice-binding protein (FfIBP) to survive and reduce damage from ice growth. The FfIBP has been cloned and over-expressed in Escherichia coli. To diagnose ice-binding mechanism, we measured thermal hysteresis (TH) activity which is numerical value of a gap between freezing and melting point as well as ice re-crystallization inhibition activity. Thermal hysteresis activity of the FfIBP was approximately 2.5℃ at 50 uM that is 10 times higher than moderately active LeIBP. Furthermore, the Ice Re-crystallization inhibition activity represent the FfIBP has ability to inhibit ice growing at low concentration 2.5 uM limit. Consequently the FfIBP was classified as a hyper-active Ice binding protein. Also, preliminary X-ray crystallography was performed to reveal the ice-binding site of FfIBP at the molecular level.
한국생물공학회 [The Korean Society for Biotechnology and Bioengineering]
설립연도
1984
분야
공학>생물공학
소개
이 법인은 생물 공학의 발전과 보급에 이바지하고, 회원 상호 간의 연구 협력과 친목을 도모함을 목적으로 한다
1. 생물공학 분야의 발전을 위한 연구 협력
2. 생물공학의 실용화를 촉진시키기 위한 산학 협동
3. 학술연구 발표회, 강연회, 연수회 등 학술활동의 개최
4. 국,영문 학술지,소식지,학술회의 Proceedings 및 학술도서의 발간
5. 생물공학 발전을 위한 정책 건의
6. 기타 국제 교류 등 본 학회의 목적 달성을 위한 제반 활동