Siegesbeckiae herba, which exerted dosedependent inhibitory effects against angiogenesis, adipogenesis, and cell adhesion. S. herba extract (SHE) exhibits an angiogenesis inhibitory effect superior to that of the EGCG from green tea leaves. Moreover, 25 ppm of SHE fraction(F5) was sufficient to block angiogenesis of the HUVEC cells morphologically. Stimulated U937 adhered to HUVEC cells, SHE at concentrations of 1, 2.5, 5, and 10 ppm suppressed U937 cell adhesion by 28, 61,70, and 158%, respectively. PMA (phorbol myristyl atetate) and different concentrations (1, 2.5, 5, and 10 ppm) of SHE-stimulated U937 adhered to the HUVEC cells. SHE at 1, 2.5, 5, and 10 ppm suppressed U937 cell adhesion by 372, 613, 633, and 720%, respectively. Different concentrations (1, 2.5, 5, and 10 ppm) of SHE stimulated U937 adhered to IL-1β and different concentrations (1, 2.5, 5, and 10 ppm) of SHE stimulated HUVEC cells. SHE suppressed U937 cell adhesion in a dosedependent manner, by percentages of 93, 95, 375, and 436%, respectively. The expression level of angiogenesis and adipogenesis-related signal molecules in the western blotting was reduced by increasing the amount of added SHE. Moreover, a diet supplemented with SHE was deemed more effective in inducing weight loss in LB mice than a representative synthetic diet drug, orlistat, which incidently caused the side effect of denuding the mice of their hair. These results indicate that S. herba may prove to be a useful anti-adipogenic compound, and these in vitro results may be reflected later under in vivo conditions.
Jong Deog KIM [ Department of Biotechnology, Research center on Anti-Obesity and Health Care, Chonnam National University, San96-1, Dun-Duk Dong, Yosu, Chonnam, 550-749, Korea. ]
Min Yong KIM [ Dept. of Refrigeration Engineering, Research center on Anti-Obesity and Health Care, Chonnam National University, San96-1, Dun-Duk Dong, Yosu, Chonnam, 550- 749, Korea. ]
한국생물공학회 [The Korean Society for Biotechnology and Bioengineering]
설립연도
1984
분야
공학>생물공학
소개
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