Earticle

초록

영어

RNA is one of promising candidates which could satisfy requirements for gene expression regulator such as scalability and modularity. There are numerous examples in nature which regulates gene expression by RNA-based mechanisms. Moreover, engineers have been producing RNA-based devices by mimicking their natural counterparts(1). We produced RNA devices which control expression level of downstream gene in response to concentration of L-tryptophan utilizing previously selected aptamer(2). The RNA devices harbor a tetracycline resistance gene, tetA, in downstream of expression platform. One of selected devices was transformed to Escherichia coli W3110 and its mutant, Δ aroG strain. Then, we measured their growth under selective condition using tetracycline. Deletion of aroG should significantly reduce intracellular tryptophan concentration since the product of aroG gene is responsible for 80% of total DS(3-Deoxy-D-arabino-heptulosonate 7-phosphate Synthase) activity in common aromatic amino acid pathway(3). Specific growth rates of WT and ΔaroG in M9(Cmp, tet 140μg/ml) were 0.16 and 0.02(h-1), respectively.

키워드

저자

• SungHo JANG [ Department of Chemical Engineering, Pohang University of Science and Technology, San 31, Hyoja-dong, Nam-gu, Pohang, Gyeongbuk, Korea, 790- 784. ]
• Sang Woo SEO [ Department of Chemical Engineering, Pohang University of Science and Technology, San 31, Hyoja-dong, Nam-gu, Pohang, Gyeongbuk, Korea, 790- 784. ]
• Gyoo Yeol JUNG [ School of Interdisciplinary Bioscience and Bioengineering and Department of Chemical Engineering, Pohang University of Science and Technology, San 31, Hyoja-dong, Nam-gu, Pohang, Gyeongbuk, Korea, 790-784. ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  한국생물공학회 학술대회

• 간기

  반년간

• 수록기간

  1985~2013

• 십진분류

  KDC 476 DDC 576