Earticle

초록

영어

The use of biocatalysts such as isolated enzymes and whole-cell systems for industrial applications requires high-yield and low-cost conversion for economical feasibility. One of the issues to be solved is to continuously supply cofactors that are often needed for complex enzymatic reactions but typically expensive. Allowing catalytic amounts of cofactors to be regenerated during the processes can provide a driving force to the thermodynamically unfavorable reactions and subsequently increase the yield and productivity of the bio-catalysis. In this study, we re-designed NADH-regeneration system of E. coli for the high-yield production of butyrate from glucose. The engineered strain showed a theoretical yield of 95% and volumetric productivity of 0.255g/l/h in a batch culture. Our work demonstrated that E. coli can be probably used as a butyrate producer, and this strategy can be broadly utilized for the production of various useful chemicals in the fields of metabolic engineering and synthetic biology.

키워드

저자

• Jae Hyung LIM [ School of Interdisciplinary Bioscience and Bioengineering , POSTECH, Pohang. ]
• Sang Woo SEO [ Department of Chemical Engineering, POSTECH, Pohang. ]
• Se Yeon KIM [ School of Interdisciplinary Bioscience and Bioengineering, POSTECH, Pohang. ]
• Gyoo Yeol JUNG [ School of Interdisciplinary Bioscience and Bioengineering and Department of Chemical Engineering, POSTECH, Pohang. ]

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간행물

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• 간행물명

  한국생물공학회 학술대회

• 간기

  반년간

• 수록기간

  1985~2013

• 십진분류

  KDC 476 DDC 576