Jae-Min PARK, Jihee YOON, Hongik KIM, Jiho MIN, Yang-Hoon KIM
언어
영어(ENG)
URL
https://www.earticle.net/Article/A174462
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원문정보
초록
영어
Chitosan (CS) nanofibers with a diameter of 150-200 nm were fabricated from mixed chitosan/poly (vinyl alcohol) (PVA) solution by the electrospinning method. The nascent CS/PVA nanofibers were treated with 0.5M NaOH solution for making stable CS nanofibers by removing PVA in aqueous conditions. Hen egg-white lysozyme was immobilized on electrospun CS nanofibers via cross-linked enzyme aggregates (CLEA) and used to effective and continuous antibacterial applications. The maximum amounts of lysozyme immobilized on the CS nanofibers were determined to be 62.3 mg/g of nanofibers under optimum condition. The immobilized lysozyme-CLEA retained more than 75.4% of its initial activity after 80 days of storage at room temperature, while the free lysozyme lost all under same condition. In addition, the immobilized lysozyme-CLEA also retained more than 92.4% activity after 50 consecutive uses. Finally, the durability of lysozyme-CLEA immobilized CS nanofibers showed 82.4% and 79.8% of bacteriostasis ratio after 10 cycles against 2 pathogenic bacteria including Staphylococcus aureus, Bacillus subtilis, respectively. These results demonstrated the lysozyme-CLEA immobilized CS nanofibers could be used as a promising material for enhanced and continuous antibacterial applications.
Jae-Min PARK [ Department of Microbiology, College of Natural Sciences, Chungbuk National University, 52 Naesudong-Ro, Heungduk-Gu, Cheongju, Chungbuk 361-763, South Korea. ]
Jihee YOON [ Graduate School of Semiconductor and Chemical Engineering, Chonbuk National University, 664-14 Deokjin-dong, 1Ga Deokjin-Gu, Jeonju 561-756, South Korea. ]
Hongik KIM [ R&D Division, Vitabio, Inc., 483 Expo-Ro, Yuseong-Gu, Daejeon 300-824, South Korea. ]
Jiho MIN [ Graduate School of Semiconductor and Chemical Engineering, Chonbuk National University, 664-14 Deokjin-dong, 1Ga Deokjin-Gu, Jeonju 561-756, South Korea. ]
Yang-Hoon KIM [ Department of Microbiology, College of Natural Sciences, Chungbuk National University, 52 Naesudong-Ro, Heungduk-Gu, Cheongju, Chungbuk 361-763, South Korea. ]
한국생물공학회 [The Korean Society for Biotechnology and Bioengineering]
설립연도
1984
분야
공학>생물공학
소개
이 법인은 생물 공학의 발전과 보급에 이바지하고, 회원 상호 간의 연구 협력과 친목을 도모함을 목적으로 한다
1. 생물공학 분야의 발전을 위한 연구 협력
2. 생물공학의 실용화를 촉진시키기 위한 산학 협동
3. 학술연구 발표회, 강연회, 연수회 등 학술활동의 개최
4. 국,영문 학술지,소식지,학술회의 Proceedings 및 학술도서의 발간
5. 생물공학 발전을 위한 정책 건의
6. 기타 국제 교류 등 본 학회의 목적 달성을 위한 제반 활동