Earticle

초록

영어

Flavonols possess antioxidant and free radical scavenging activity in foods, with myricetin being one of the most active. In plant, kaempferol converts to myricetin by 3’, 5’-hydroxylase which is cytochrome P450. The cytochrome P450s belong to a family of heme containing monooxygenases and catalyze diverse reaction, such as hydroxylation, epoxidation and O-demethylation. CYP102D1, bi-functional cytochrome P450:NADPH-P450 reductase, from Streptomyces avermitilis can hydroxylate the saturated and unsaturated fatty acids. First, three positions, which are L84A/F96V/L448A, were done saturation mutagenesis to increase the size of active site pocket and substrate access channel. Second, A273/G274/T277 was selected among the amino acid within 7Å from heme to saturated mutagenesis. For high throughput screening, aldehyde sensing method was applied with schiff’s test. Finally, we screened six CYP102 mutants towards permethylated kaempferol using schiff’s reagent agar plate assay and expressed.

키워드

저자

• Byeo-Ri AN [ Dept. of Chemical and Biological Engineering, Seoul National University, Seoul, Korea. ]
• Kwon-young CHOI [ Dept. of Chemical and Biological Engineering, Seoul National University, Seoul, Korea. ]
• Eunok JUNG [ Dept. of Chemical and Biological Engineering, Seoul National University, Seoul, Korea. ]
• Da-Hye JUNG [ Dept. of Chemical and Biological Engineering, Seoul National University, Seoul, Korea. ]
• Wonji CHOI [ Dept. of Chemical and Biological Engineering, Seoul National University, Seoul, Korea. ]
• Byung-Gee KIM [ Dept. of Chemical and Biological Engineering, Seoul National University, Seoul, Korea. ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  한국생물공학회 학술대회

• 간기

  반년간

• 수록기간

  1985~2013

• 십진분류

  KDC 476 DDC 576