Earticle

초록

영어

Enantioselective kinetic resolution of recombinant epoxide hydrolase (EH) from a marine fish, Mugil cephalus, was investigated. The lyophilized recombinant whole-cell E. coli expressing the M. cephalus EH gene was used as the biocatalyst. The problem of instability and low solubility of styrene oxide substaret was overcome by using an organic solvent as the reaction medium. The effects of water contents, cell concentration, nature of solvent, adding the various lyoprotectants and substrate concentration on activity, stability and enantioselectivity of EH in an organic solvent were optimized. Various organic solvents with log P value between - 0.7 to 4.5 were screened as the reaction solvent. To select the most suitable lyoprotectant, the cells were lyophilized in the presence of various lyoprotectants and detergents. Enantioselective kinetic resolution of 20 mM styrene oxide was carried out in a shaking incubator at 30 ℃ and 250 rpm, and enantiopure (S)-strene oxide with 98% ee was readily obtained.

키워드

저자

• Ok Kyung LEE [ Department of Chemical Engineering, Kyung Hee University, Gyeonggi-do 446-701, Korea. ]
• Sung Hee CHOI [ Department of Chemical Engineering, Kyung Hee University, Gyeonggi-do 446-701, Korea. ]
• Yoon Ju JO [ Department of Chemical Engineering, Kyung Hee University, Gyeonggi-do 446-701, Korea. ]
• Eun Yeol LEE [ Department of Chemical Engineering, Kyung Hee University, Gyeonggi-do 446-701, Korea. ]

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발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  한국생물공학회 학술대회

• 간기

  반년간

• 수록기간

  1985~2013

• 십진분류

  KDC 476 DDC 576