Hee Sook KIM, Hwan Hee PARK, Yoo Jung PARK, Eun Yeol LEE
언어
영어(ENG)
URL
https://www.earticle.net/Article/A174416
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원문정보
초록
영어
PolyM-specific, polyG-specific and polyMG-specific alginate lyase were cloned by using PCR with homologous nucleotide sequences-based primers from various marine bacteria, previously. Overexpressed alginate lyase exhibited substrate specificity and showed low identity of protein sequence each other. The recombinant alginate lyases were homology modeled on the crystal structures as templates. Alginate oligomers produced from the recombinant alginate lyases with different substrate specificities were analyzed by FPLC and high-field 1H nuclear magnetic resonance (NMR) spectroscopy. Protein structures of substrate-specific alginate lyase were analyzed to have a b-jelly roll fold, ( α / α ) 6 barrel fold or parallel β-helix. Acknowledgement: This work was supported by New &Renewable Energy R&D program(20093020090020) and Korea Institute for Advancement in Technology (KIAT) through the Workforce Development Program in Strategic Technology under the Korea Ministry of Knowledge Economy(MKE).
키워드
Substrate specific alginate lyaseSubstrate specific alginate lyasehomology modelled
저자
Hee Sook KIM [ Department of Food Science and Biotechnology, Kyungsung University, Busan 608-736, Korea. ]
Hwan Hee PARK [ Department of Food Science and Biotechnology, Kyungsung University, Busan 608-736, Korea. ]
Yoo Jung PARK [ Department of Food Science and Biotechnology, Kyungsung University, Busan 608-736, Korea. ]
Eun Yeol LEE [ Department of Chemical Engineering, Kyung Hee University, Gyeonggi-do 446-701, Korea. ]
한국생물공학회 [The Korean Society for Biotechnology and Bioengineering]
설립연도
1984
분야
공학>생물공학
소개
이 법인은 생물 공학의 발전과 보급에 이바지하고, 회원 상호 간의 연구 협력과 친목을 도모함을 목적으로 한다
1. 생물공학 분야의 발전을 위한 연구 협력
2. 생물공학의 실용화를 촉진시키기 위한 산학 협동
3. 학술연구 발표회, 강연회, 연수회 등 학술활동의 개최
4. 국,영문 학술지,소식지,학술회의 Proceedings 및 학술도서의 발간
5. 생물공학 발전을 위한 정책 건의
6. 기타 국제 교류 등 본 학회의 목적 달성을 위한 제반 활동