Earticle

초록

영어

Regulatory noncoding RNAs, such as small regulatory RNAs (sRNAs), are fast-acting and less-energy-consuming regulator widely used in many organisms to precisely control gene expression together with transcriptional control, and therefore they could be a vulnerable regulator for gene expression control. Here we present an approach for constructing synthetic small regulatory RNAs for controlling gene expression. We developed synthetic small regulatory RNAs repressing the translation of DsRed2 mRNA at various levels and also constructed three different sRNAs for the mRNAs of LuxR, AraC, and KanR without cross-reactivity. The results suggest that gene expression can be finetuned by designed artificial small RNAs. The possibility of designing small regulatory RNAs may facilitate the development of precisely regulated synthetic circuits. [This work was supported by the Intelligent Synthetic Biology Center of Global Frontier Project (2011-0031963) funded by the Ministry of Education, Science and Technology, Korea and by the World Class University program (R32-2008-000-10142-0) of the MEST.]

키워드

저자

• Seung Min YOO [ Dept. of Chemical and Biomolecular Engineering, KAIST, Daejeon, 305.701 ]
• Dokyun NA [ Dept. of Chemical and Biomolecular Engineering, KAIST, Daejeon, 305.701 ]
• Sang Yup LEE [ Dept. of Chemical and Biomolecular Engineering, KAIST, Daejeon, 305.701 ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  한국생물공학회 학술대회

• 간기

  반년간

• 수록기간

  1985~2013

• 십진분류

  KDC 476 DDC 576