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초록

영어

Riboswitches are a powerful synthetic tool for gene regulation in molecular biology. They control gene expression by adopting alternative RNA structures in the presence or absence of small ligands. Most of the riboswitches are prokaryotic and here we describe a novel concept in gene regulation through eukaryotic intragenic riboswitch. In this investigation, we constructed a chimeric mRNA that contain theophylline aptamer after the start codon and studied the effect on translational gene expression in S.cerevisiae. It was observed on addition of theophylline, the strong secondary structure was formed at the mRNA 5’ proximal coding region caused by the theophylline aptamer, thereby disturbing the translation process. Consequently, it led to the decrease in the translational gene expression level. We also observed same effect using the tetracycline aptamer in this system. This new gene off-system can be used to simultaneously regulate the expression level of xylose reductase (XR) and xylitol dehydrogenase (XDH) from P.stipites, as well as reporter protein such as yellow fluorescent protein. The enzymes, XR and XDH are important in the bioethanol production by xylose fermentation and finding the optimal ratio of the enzymes in S.cerevisiae is critical for the success of the process. Our eukaryotic intragenic riboswitch can be used in addressing the bottle neck of xylose metabolic flux between xylose reductase and xylitol dehydrogenase in S.cerevisiae. This work was supported by the Advanced Biomass R&D Center (ABC-2010-0029737) through the National Research Foundation of Korea funded by the Ministry of Education, Science, and Technology.

키워드

저자

• Ji-Hyeon SHIN [ Department of Biotechnology, Yonsei University,Seoul, 120-749. ]
• Jong-Shik SHIN [ Department of Biotechnology, Yonsei University,Seoul, 120-749. ]

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간행물

표지보기 관심저널 등록

• 간행물명

  한국생물공학회 학술대회

• 간기

  반년간

• 수록기간

  1985~2013

• 십진분류

  KDC 476 DDC 576