Earticle

초록

영어

Industrialization has resulted in emission of large amount of carbon monoxide into the atmosphere, which is hazardous to living organisms. Therefore conversion of CO to non toxic materials is highly essential. Hydrogen production from CO will be a value added contribution in replacing fossil fuels, as hydrogen has enormous potential as future energy due to its abundance and non-polluting nature. Citrobacter amalonaticus Y19, a facultative anaerobic bacterium, isolated by Jung et al. (2002) [1], was characterized for hydrogen production from the toxic carbon monoxide gas. Contig genome analysis of this microorganism revealed that cooFSCTJhypB genes are involved in expressing functional carbon monoxide dehydrogenase (CODH). These genes were heterologously overexpressed in E.coli BL21 (DE3) by cloning in pACYCDuet vector. This resulted in an enzyme activity of 269 μmol CO min-1mg protein-1, which is comparatively higher than the CODH activity in wild type Y19 [2].

키워드

저자

• Balaji SUNDARA SEKAR [ Department of Chemical and Biochemical Engineering, Pusan National University, Busan 609-735, Republic of Korea. ]
• Jungeun LEE [ Department of Chemical and Biochemical Engineering, Pusan National University, Busan 609-735, Republic of Korea. ]
• Eunhee SEOL [ Department of Chemical and Biochemical Engineering, Pusan National University, Busan 609-735, Republic of Korea. ]
• Ritam SARKAR [ Department of Chemical and Biochemical Engineering, Pusan National University, Busan 609-735, Republic of Korea. ]
• Sunghoon PARK [ Department of Chemical and Biochemical Engineering, Pusan National University, Busan 609-735, Republic of Korea. ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  한국생물공학회 학술대회

• 간기

  반년간

• 수록기간

  1985~2013

• 십진분류

  KDC 476 DDC 576