Hyo-Ji KIM, Hye-Yun PARK, Mi-Young CHEON, Yong-Il HWANG, Sun-Uk CHOI
언어
영어(ENG)
URL
https://www.earticle.net/Article/A174393
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원문정보
초록
영어
Actinomycetes are versatile producers of a variety of secondary metabolites, including more than two-thirds of all antibiotics employed in the fields of human medicine and agriculture. Among the known actinomycetes, only a few species are known to cause scab diseases in economically important root and tuber crops. Effective transformation procedure to facilitate molecular genetic studies of Streptomyces acidiscabies producing thaxtomin, which causes scab diseases in the economically important root and tuber crops was established via transconjugation from Escherichia coli ET12567 using an ØC31-derived integration vector, pSET152, harboring the oriT and attP fragments. Greatest number of exconjugants was achieved on MS medium containing 50 mM MgCl2 without heat treatment of spores and E. coli cells. Additionally, the integration site, attB, of the genome of S. acidiscabies exhibited the highest degree of homology with S. avermitilis and its chromosomal location was found to exist as a single attB site within an open reading frame coding for a pirin homolog similar to those identified in other actinomycetes. [This research was supported by Basic Science Research Program through the National Research Foundation of Korea(NRF) funded by the Ministry of Education, Science and Technology(2010-0008443)]
키워드
attB siteMgCl2Streptomyces acidiscabiesintergeneric conjugal transfer
저자
Hyo-Ji KIM [ Dept. of Food Science and Biotechnology, Kyungnam University, Changwon 631-701. ]
Hye-Yun PARK [ Dept. of Food Science and Biotechnology, Kyungnam University, Changwon 631-701. ]
Mi-Young CHEON [ Dept. of Food Science and Biotechnology, Kyungnam University, Changwon 631-701. ]
Yong-Il HWANG [ Dept. of Food Science and Biotechnology, Kyungnam University, Changwon 631-701. ]
Sun-Uk CHOI [ Dept. of Food Science and Biotechnology, Kyungnam University, Changwon 631-701. ]
한국생물공학회 [The Korean Society for Biotechnology and Bioengineering]
설립연도
1984
분야
공학>생물공학
소개
이 법인은 생물 공학의 발전과 보급에 이바지하고, 회원 상호 간의 연구 협력과 친목을 도모함을 목적으로 한다
1. 생물공학 분야의 발전을 위한 연구 협력
2. 생물공학의 실용화를 촉진시키기 위한 산학 협동
3. 학술연구 발표회, 강연회, 연수회 등 학술활동의 개최
4. 국,영문 학술지,소식지,학술회의 Proceedings 및 학술도서의 발간
5. 생물공학 발전을 위한 정책 건의
6. 기타 국제 교류 등 본 학회의 목적 달성을 위한 제반 활동