Du-Ho SON, Nan-Seul KIM, Sang-eun LEE, Yong-Il HWANG, Sun-Uk CHOI
언어
영어(ENG)
URL
https://www.earticle.net/Article/A174392
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원문정보
초록
영어
Streptomyces natalensis produces natamycin, predominantly strong antifungal agent, inhibiting the growth of both yeasts and molds and preventing formation of aflatoxin in filamentous fungi. Due to the low toxicity of natamycin to mammalian cells compared to other antifungal compounds, it has found many applications in the treatment of various fungal diseases. Also, AfsR acts as a transcriptional factor in both the regulation of secondary metabolism and morphological differentiation in Streptomyces strains. In this study, PCR using the primers designed from the two highly conserved regions of the previously studied AfsR of Streptomyces strains gave 763- bp products. The sequence of this product had a high similarity to the expected region of an afsR gene. An intact 2,943 bp of afsR gene was cloned from S. natalensis by genomic Southern hybridization with PCR product as a probe, and then studied. To identify the function of the cloned afsR gene, it was inserted into pSET152ET to construct a high expression vector, and then was introduced into Streptomyces strains. The exconjugant including the high expression vector of afsR was constructed, and its phenotype was compared with the control strain. [This research was supported by Basic Science Research Program through the National Research Foundation of Korea(NRF) funded by the Ministry of Education, Science and Technology (2010-0008443)]
키워드
afsRStreptomyces natalensisnatamycin
저자
Du-Ho SON [ Department of Food Science and Biotechnology, Kyungnam University, Changwon, 631-701, Republic of Korea. ]
Nan-Seul KIM [ Department of Food Science and Biotechnology, Kyungnam University, Changwon, 631-701, Republic of Korea. ]
Sang-eun LEE [ Department of Food Science and Biotechnology, Kyungnam University, Changwon, 631-701, Republic of Korea. ]
Yong-Il HWANG [ Department of Food Science and Biotechnology, Kyungnam University, Changwon, 631-701, Republic of Korea. ]
Sun-Uk CHOI [ Department of Food Science and Biotechnology, Kyungnam University, Changwon, 631-701, Republic of Korea. ]
한국생물공학회 [The Korean Society for Biotechnology and Bioengineering]
설립연도
1984
분야
공학>생물공학
소개
이 법인은 생물 공학의 발전과 보급에 이바지하고, 회원 상호 간의 연구 협력과 친목을 도모함을 목적으로 한다
1. 생물공학 분야의 발전을 위한 연구 협력
2. 생물공학의 실용화를 촉진시키기 위한 산학 협동
3. 학술연구 발표회, 강연회, 연수회 등 학술활동의 개최
4. 국,영문 학술지,소식지,학술회의 Proceedings 및 학술도서의 발간
5. 생물공학 발전을 위한 정책 건의
6. 기타 국제 교류 등 본 학회의 목적 달성을 위한 제반 활동