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Characterization of a global regulatory gene, afsR, derived from Streptomyces natalensis

첫 페이지 보기
  • 발행기관
    한국생물공학회 바로가기
  • 간행물
    한국생물공학회 학술대회 바로가기
  • 통권
    2012 춘계학술대회 및 국제심포지움 (2012.04)바로가기
  • 페이지
    pp.165-165
  • 저자
    Du-Ho SON, Nan-Seul KIM, Sang-eun LEE, Yong-Il HWANG, Sun-Uk CHOI
  • 언어
    영어(ENG)
  • URL
    https://www.earticle.net/Article/A174392

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원문정보

초록

영어
Streptomyces natalensis produces natamycin, predominantly strong antifungal agent, inhibiting the growth of both yeasts and molds and preventing formation of aflatoxin in filamentous fungi. Due to the low toxicity of natamycin to mammalian cells compared to other antifungal compounds, it has found many applications in the treatment of various fungal diseases. Also, AfsR acts as a transcriptional factor in both the regulation of secondary metabolism and morphological differentiation in Streptomyces strains. In this study, PCR using the primers designed from the two highly conserved regions of the previously studied AfsR of Streptomyces strains gave 763- bp products. The sequence of this product had a high similarity to the expected region of an afsR gene. An intact 2,943 bp of afsR gene was cloned from S. natalensis by genomic Southern hybridization with PCR product as a probe, and then studied. To identify the function of the cloned afsR gene, it was inserted into pSET152ET to construct a high expression vector, and then was introduced into Streptomyces strains. The exconjugant including the high expression vector of afsR was constructed, and its phenotype was compared with the control strain. [This research was supported by Basic Science Research Program through the National Research Foundation of Korea(NRF) funded by the Ministry of Education, Science and Technology (2010-0008443)]

키워드

afsR Streptomyces natalensis natamycin

저자

  • Du-Ho SON [ Department of Food Science and Biotechnology, Kyungnam University, Changwon, 631-701, Republic of Korea. ]
  • Nan-Seul KIM [ Department of Food Science and Biotechnology, Kyungnam University, Changwon, 631-701, Republic of Korea. ]
  • Sang-eun LEE [ Department of Food Science and Biotechnology, Kyungnam University, Changwon, 631-701, Republic of Korea. ]
  • Yong-Il HWANG [ Department of Food Science and Biotechnology, Kyungnam University, Changwon, 631-701, Republic of Korea. ]
  • Sun-Uk CHOI [ Department of Food Science and Biotechnology, Kyungnam University, Changwon, 631-701, Republic of Korea. ]

참고문헌

자료제공 : 네이버학술정보

간행물 정보

발행기관

  • 발행기관명
    한국생물공학회 [The Korean Society for Biotechnology and Bioengineering]
  • 설립연도
    1984
  • 분야
    공학>생물공학
  • 소개
    이 법인은 생물 공학의 발전과 보급에 이바지하고, 회원 상호 간의 연구 협력과 친목을 도모함을 목적으로 한다 1. 생물공학 분야의 발전을 위한 연구 협력 2. 생물공학의 실용화를 촉진시키기 위한 산학 협동 3. 학술연구 발표회, 강연회, 연수회 등 학술활동의 개최 4. 국,영문 학술지,소식지,학술회의 Proceedings 및 학술도서의 발간 5. 생물공학 발전을 위한 정책 건의 6. 기타 국제 교류 등 본 학회의 목적 달성을 위한 제반 활동

간행물

  • 간행물명
    한국생물공학회 학술대회
  • 간기
    반년간
  • 수록기간
    1985~2013
  • 십진분류
    KDC 476 DDC 576

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