Earticle

초록

영어

For a long time, enzyme-linked immunosorbent assay (ELISA) has been widely used for protein interaction asssay as well as various diagnostic systems. In conventional ELISA system, use of HRP conjugate antibody probes are required for detection signaling, however the low sensitivity of HRP reaction and use of high-cost antibody probes are considered as limitations. Recently various non-antibody scaffold proteins which have relatively small size (<100 aa) and rigid core structure have been developed as an alternative antibody. Compared with traditional antibody molecules, scaffold proteins have several beneficial points including i) simple production with high production yield by Escherichia coli host system, ii) easy engineering against various target materials with high affinity, and iii) high stability under the unfavorable environments which make scaffold proteins suitable for diagnostic system. Here, we developed a new fluorescence-linked immunosorbent assay (FLISA) system using GFP-fused scaffold protein allowing simple and sensitive detection of target molecules. In this system, GFP fused scaffold proteins could be produced in cytoplasm of E. coli with high productivity and solubility, and also successfully purified with high recovery yield. The purified protein maintained high affinity to the target molecules, and strong fluorescence intensity of GFP could be detected.

키워드

저자

• Gu Min JEONG [ Dept. of Chemical & Biomolecular Engineering, KAIST, Daejeon, 305-701. ]
• Ki Jun JEONG [ Dept. of Chemical & Biomolecular Engineering, KAIST, Daejeon, 305-701. ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  한국생물공학회 학술대회

• 간기

  반년간

• 수록기간

  1985~2013

• 십진분류

  KDC 476 DDC 576