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초록

영어

In the previous work, we reported semiconductor nanowires synthesized by VLS mechanism could be applied for matrix-free laser desorption/ionization ? time of flight analysis called nanowire-assisted laser desorption/ionization mass spectroscopy (NALDI-MS). By using this method, the quantitative analysis of small molecule could be achieved without adding organic matrix molecules. In order to apply TiO2 nanowires to NALDI-TOF MS, the nanowires were synthesized by mild hydrothermal process directly on the surface of Ti plate. For this study, the sample target plate was patterned to have 81 TiO2 nanowire zones on the surface. The morphology of the synthesized TiO2 nanowires were observed to be a diameter of 30 nm by SEM, and the crystal structure was determined to be a mixture of anatase and rutile phases by XRD. The feasibility of applying the synthesized TiO2 nanowires to NALDI-TOF MS was tested by the quantitative analysis of bradykinin fragments 1-7 and P14R synthetic peptide. Mass spectrometric analyses of the compounds mainly showed [M+H]+ and [M+K]+ ion peaks by using TiO2 nanowires, and the sums of the two peaks was considered to be signals for the quantitative analysis. The summed signals from bradykinin fragments 1-7 were exponentially increased with a correlation coefficient of 0.997 in a concentration range of 5 ? 100 nmol/mL. Similarly, P14R synthetic peptide showed exponentially increased signals in the same concentration range. The results show that the synthetic TiO2 nanowires could be applied to laser desorption/ionization MS, and the quantitative analysis of small compounds by using top-down synthesized TiO2 nanowires were feasible.

키워드

저자

• Jo-Il KIM [ Department of Materials Science and Engineering, Yonsei University, Seoul, Korea. ]
• Jae-Chul PYUN [ Department of Materials Science and Engineering, Yonsei University, Seoul, Korea. ]

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간행물

표지보기 관심저널 등록

• 간행물명

  한국생물공학회 학술대회

• 간기

  반년간

• 수록기간

  1985~2013

• 십진분류

  KDC 476 DDC 576