Immunoassay has been widely used for the detection of target analytes in complex mixtures such as blood by using highly specific antigenantibody interactions. In this work, antibodies against a target analyte was immobilized to magnetic beads and the change of surface charge of a magnetic bead was measured after the binding of a target protein by using a zeta potential analyzer. For the feasibility test of the zeta potential analysis for immunoassays, horseradish peroxidase (HRP) was used as a model target analyte. The application to medical diagnosis was demonstrated by detection of C-reactive protein (CRP) which is known to be a biomarker for inflammatory diseases.
키워드
zetapotentialnon-labeledimmunoassay
저자
Eun-Hang LEE [ Dept. of Material Science and Engineering, Yonsei university, Seoul, 120-749. ]
Ju-Kyung LEE [ Dept. of Material Science and Engineering, Yonsei university, Seoul, 120-749. ]
Yong-Hee LEE [ Dept. of Material Science and Engineering, Yonsei university, Seoul, 120-749. ]
Jae-Chul PYUN [ Dept. of Material Science and Engineering, Yonsei university, Seoul, 120-749. ]
한국생물공학회 [The Korean Society for Biotechnology and Bioengineering]
설립연도
1984
분야
공학>생물공학
소개
이 법인은 생물 공학의 발전과 보급에 이바지하고, 회원 상호 간의 연구 협력과 친목을 도모함을 목적으로 한다
1. 생물공학 분야의 발전을 위한 연구 협력
2. 생물공학의 실용화를 촉진시키기 위한 산학 협동
3. 학술연구 발표회, 강연회, 연수회 등 학술활동의 개최
4. 국,영문 학술지,소식지,학술회의 Proceedings 및 학술도서의 발간
5. 생물공학 발전을 위한 정책 건의
6. 기타 국제 교류 등 본 학회의 목적 달성을 위한 제반 활동