Su-Jin OH, Ka-Young LEE, Kyung-Ho LEE, Dong-Myung KIM
언어
영어(ENG)
URL
https://www.earticle.net/Article/A174383
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원문정보
초록
영어
The use of magnetic bead-immobilized DNA as movable template for cell-free protein synthesis has been investigated. Magnetic microbeads containing chemically conjugated plasmids were used to direct cell-free protein synthesis, so that protein generation could be readily programmed, reset and reprogrammed. Protein synthesis by using this approach could be ON/OFF-controlled through repeated addition and removal of the microbead-conjugated DNA and employed in sequential expression of different genes in a same reaction mixture. Since the incubation periods of individual template plasmids are freely controllable, relative expression levels of multiple proteins can be tuned to desired levels. We expect that the presented results will find wide application to the flexible design and execution of synthetic pathways in cell-free chassis.
키워드
Cell-free protein synthesisImmobilization of DNAMagnetic particles
저자
Su-Jin OH [ Department of Fine Chemical Engineering and Applied Chemistry, Chungnam National University, Daejeon 305-764 ]
Ka-Young LEE [ Department of Fine Chemical Engineering and Applied Chemistry, Chungnam National University, Daejeon 305-764 ]
Kyung-Ho LEE [ Interdisciplinary Program for Nano-Technology, Chungnam National University, Daejeon 305-764. ]
Dong-Myung KIM [ Department of Fine Chemical Engineering and Applied Chemistry, Chungnam National University, Daejeon 305-764 ]
한국생물공학회 [The Korean Society for Biotechnology and Bioengineering]
설립연도
1984
분야
공학>생물공학
소개
이 법인은 생물 공학의 발전과 보급에 이바지하고, 회원 상호 간의 연구 협력과 친목을 도모함을 목적으로 한다
1. 생물공학 분야의 발전을 위한 연구 협력
2. 생물공학의 실용화를 촉진시키기 위한 산학 협동
3. 학술연구 발표회, 강연회, 연수회 등 학술활동의 개최
4. 국,영문 학술지,소식지,학술회의 Proceedings 및 학술도서의 발간
5. 생물공학 발전을 위한 정책 건의
6. 기타 국제 교류 등 본 학회의 목적 달성을 위한 제반 활동