Caffeine, aspirin, and tetracycline have been used in human life as diuretic, analgesic, and antibiotic. These pharmaceuticals have been detected in surface water resulting in the aquatic ecosystems confusion and human health change. In this study, it was analyzed specific stress responses caused by pharmaceuticals such as caffeine (890mg/l to 2225mg/l), aspirin (620mg/l to 1550mg/l) and tetracycline (180mg/l to 450mg/l) using four different stress responsive genes in Escherichia coli, i.e. fabA, grpE, katG and recA. The expression levels of these genes were quantified by semi-quantitative reverse transcription-PCR. It was found that four genes have shown different responsive patterns when E. coli cultures were under stressful conditions caused by caffeine, aspirin, tetracycline, respectively. Therefore, in this study, the stress responsive effects caused by these pharmaceuticals and the extent of each stress response can be analyzed using the expression levels and patterns of different stress responsive genes
키워드
caffeineaspirintetracycline
저자
Nam-Hui HONG [ Department of Bioprocess Engineering, Chonbuk National University, 567, Baekje-Daero, Duckjin-gu, Jeonju, Jeonbuk, 561-756. ]
한국생물공학회 [The Korean Society for Biotechnology and Bioengineering]
설립연도
1984
분야
공학>생물공학
소개
이 법인은 생물 공학의 발전과 보급에 이바지하고, 회원 상호 간의 연구 협력과 친목을 도모함을 목적으로 한다
1. 생물공학 분야의 발전을 위한 연구 협력
2. 생물공학의 실용화를 촉진시키기 위한 산학 협동
3. 학술연구 발표회, 강연회, 연수회 등 학술활동의 개최
4. 국,영문 학술지,소식지,학술회의 Proceedings 및 학술도서의 발간
5. 생물공학 발전을 위한 정책 건의
6. 기타 국제 교류 등 본 학회의 목적 달성을 위한 제반 활동