Listeria monocytogenes is a facultative anaerobe, gram-positive bacterium that is isolated from the soil, vegetables, and wild or domestic animals. Listeria occurs predominantly in the elderly, immunocompromised patients, pregnant women and newborns. Infection by L. monocytogenes causes the disease listeriosis in humans that may meningitis, meningoencephalitis, brain abscess, pyogenic arthritis, osteomylitis, and liver abscesses. When this bacteria is present within a host organism quorum sensing cause the up regulation of several virulence genes. The prfA is the main activator of Listeria virulence gene expression in blood. Therefore, we have developed a new approach for detection of listeriosis using DNA aptamers. We selected target protein (prfA) to regulator of virulence gene expression and performed 10 cycle of SELEX (systematic evolution of ligands by exponential enrichment). Using real-time PCR and surface plasmon resonance (SPR), we tested the binding effects of aptamers. After aptamers obtained 9 cycle of selection demonstrated the high affinity and specific binding aptamers with prfA. In conclusion, aptasensor based on specific prfA binding aptamers can be effectively applied for the rapid and sensitive detection of L. monocytogenes from the contaminated environments such as corrupt food products, polluted drinking water, and stable vegetables.
키워드
rapid kitListeriosisDNA aptamerprfA
저자
Sang-Hee LEE [ Department of Microbiology, College of Natural Sciences, Chungbuk National University, 52 Naesudong-Ro, Heungduk-Gu, Cheongju, Chungbuk 361-763, South Korea. ]
Hyun-Ju UM [ Department of Microbiology, College of Natural Sciences, Chungbuk National University, 52 Naesudong-Ro, Heungduk-Gu, Cheongju, Chungbuk 361-763, South Korea. ]
Yang-Hoon KIM [ Department of Microbiology, College of Natural Sciences, Chungbuk National University, 52 Naesudong-Ro, Heungduk-Gu, Cheongju, Chungbuk 361-763, South Korea. ]
한국생물공학회 [The Korean Society for Biotechnology and Bioengineering]
설립연도
1984
분야
공학>생물공학
소개
이 법인은 생물 공학의 발전과 보급에 이바지하고, 회원 상호 간의 연구 협력과 친목을 도모함을 목적으로 한다
1. 생물공학 분야의 발전을 위한 연구 협력
2. 생물공학의 실용화를 촉진시키기 위한 산학 협동
3. 학술연구 발표회, 강연회, 연수회 등 학술활동의 개최
4. 국,영문 학술지,소식지,학술회의 Proceedings 및 학술도서의 발간
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