Earticle

초록

영어

3-hydroxypropionic acid (3-HP), an industrially important platform chemical is used as a precursor for many commercially important chemicals. Recently, the production of 3-HP from glycerol was demonstrated in recombinant K. pneumoniae without the addition of expensive coenzyme B12 [1]. 3-HP production in K. pneumoniae is always associated with the production of 1,3-propanediol and lactic acid due to the generation of large amounts of NADH [2]. To enhance 3-HP yield on glycerol by regenerating NAD+, nitrate was supplemented in the culture medium. But this resulted in significant decrease in 3-HP production and majority of carbon was utilized for cell biomass generation, due to the presence of undesirable anaerobic respiration pathway. To overcome this limitation, a novel recombinant strain, Klebsiella pneumoniae DdhaTDglpK (puuC) was developed. This strain significantly increased 3-HP production at a maximum of 22.2 g/L in the presence of nitrate under anaerobic condition with a yield on glycerol at 0.35 mol/mol.

키워드

저자

• Somasundar ASHOK [ Department of Chemical and Biomolecular Engineering, Pusan National University, Busan 609-735, South Korea. ]
• Yeounjoo KO [ Department of Chemical and Biomolecular Engineering, Pusan National University, Busan 609-735, South Korea. ]
• Subramanian Mohan RAJ [ Department of Chemical and Biomolecular Engineering, Pusan National University, Busan 609-735, South Korea. ]
• Mugesh SANKARANARAYANAN [ Department of Chemical and Biomolecular Engineering, Pusan National University, Busan 609-735, South Korea. ]
• Sunghoon PARK [ Department of Chemical and Biomolecular Engineering, Pusan National University, Busan 609-735, South Korea. ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  한국생물공학회 학술대회

• 간기

  반년간

• 수록기간

  1985~2013

• 십진분류

  KDC 476 DDC 576