Jae Hwan JUNG, Seok Jin CHOI, Byung Hyun PARK, Tae Seok SEO
언어
영어(ENG)
URL
https://www.earticle.net/Article/A174344
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원문정보
초록
영어
In this study, we demonstrated a novel method of reverse transcriptasepolymerase chain reaction (RT-PCR) using a rotary system, called Rotary Genetic Analyzer, for detecting influenza A virus with high speed. The Rotary Genetic Analyzer consists of three parts including a disposable plastic PCR microchip, thermal blocks for temperature control, and a stepper motor for precise rotating of the chip. A disposable RT-PCR microchip was fabricated by glass-PDMS hybrid with 1 ㎕ reaction volume. Three thermal blocks fitted on the rotary stage, and the temperature of each block was correspondent to the PCR thermal cycling, namely 95 ℃(denaturing), 58 ℃(annealing), 72 ℃ (extension). A stepper motor were positioned at the center and could rotate the PCR chip at each heater block as designed angle and time by LabVIEWTM. The generated amplicons by the rotary system were analyzed on a capillary electrophoretic microdevice. Target amplification was performed with only 25 min time and limit of detection was 12 ag of influenza A H3N2 virus. In addition, multiplex detection was performed confirming three influenza A virus (H1N1, H3N2, and H5N1) simultaneously.
키워드
Rotary genetic analyzerReverse transcription polymerase chain reactionInfluenza A virusMultiplex detectionmicrochip
저자
Jae Hwan JUNG [ Department of Chemical and Biomolecular Engineering (BK21 program) and Institute for the BioCentury, KAIST, Daejeon, KOREA. ]
Seok Jin CHOI [ Department of Chemical and Biomolecular Engineering (BK21 program) and Institute for the BioCentury, KAIST, Daejeon, KOREA. ]
Byung Hyun PARK [ Department of Chemical and Biomolecular Engineering (BK21 program) and Institute for the BioCentury, KAIST, Daejeon, KOREA. ]
Tae Seok SEO [ Department of Chemical and Biomolecular Engineering (BK21 program) and Institute for the BioCentury, KAIST, Daejeon, KOREA. ]
한국생물공학회 [The Korean Society for Biotechnology and Bioengineering]
설립연도
1984
분야
공학>생물공학
소개
이 법인은 생물 공학의 발전과 보급에 이바지하고, 회원 상호 간의 연구 협력과 친목을 도모함을 목적으로 한다
1. 생물공학 분야의 발전을 위한 연구 협력
2. 생물공학의 실용화를 촉진시키기 위한 산학 협동
3. 학술연구 발표회, 강연회, 연수회 등 학술활동의 개최
4. 국,영문 학술지,소식지,학술회의 Proceedings 및 학술도서의 발간
5. 생물공학 발전을 위한 정책 건의
6. 기타 국제 교류 등 본 학회의 목적 달성을 위한 제반 활동