Earticle

초록

영어

Sacchromyces cerevisiae contains cell organelles such as vacuoles correspond to lysosomes in higher eukaryotes. Lysosomes have antimicrobial activity and anticancerous effect and those are not silent organelles, but dynamic organelles in which lysosomal enzymes are easily integrated when exposed to stressful conditions, especially oxidative. In this study, we selected a protein that aminopeptidase Y (APY) conspicuously was increased than other proteins in oxidative stress, and we cloned, over-expressed and purified the APY gene production form S. cerevisiae. As a result, cloned S. cerevisiae had antimicrobial effects more than normal cells, besides those were increased more over by galactose induction. We found that anticancerous effects of lysosomes isolated from recombinant S. cerevisiae were increased on cancer cells such as HeLa cells and melanocyte in vitro assay. In addition, bacterial growth in blood was inhibited by lysosomes when we injected lysosomes for therapy agents of bacterimia in rat model. Therefore, increase in endogenous levels of lysosomes and their activity following genetic modification may have various applications as antimicrobial agent and apoptosis-inducing materials for cancer cells and consequently in may be possible to use the organelles for improving in vitro and in vivo functions.

키워드

저자

• Jihee YOON [ Graduate School of Semiconductor and Chemical Engineering, Chonbuk National University, 664-14 Deokjin-dong, 1Ga Deokjin-Gu Jeonju 561-756, South Korea. ]
• Yang-Hoon KIM [ Department of Microbiology, Chungbuk National University, 410 Sungbong-Ro, Heungduk-Gu, Cheongju 361-763, South Korea. ]
• Jiho MIN [ Graduate School of Semiconductor and Chemical Engineering, Chonbuk National University, 664-14 Deokjin-dong, 1Ga Deokjin-Gu Jeonju 561-756, South Korea. ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  한국생물공학회 학술대회

• 간기

  반년간

• 수록기간

  1985~2013

• 십진분류

  KDC 476 DDC 576