Earticle

초록

영어

Advances in custom de novo DNA synthesis technology have propelled the progress of modern biotechnology, embracing the recent emergence of synthetic biology. However, present cell-based protocols for the translation of synthesized genes are time and labor intensive, and thus limiting the throughput of creating protein molecules. Herein, we demonstrate a seamless consolidation of PCR-based synthetic gene assembly with fully automated cell-free protein synthesis for direct translation of sequence information into purified proteins. Chemically synthesized gene fragments were PCR-assembled, and incubated in the ExiProgenTM system (BIONEER, Korea) for automated, sequential cell-free expression and purification of the target proteins. Starting from assembled PCR products, 16 different proteins were successfully obtained in purified forms within 7 hours without manual intervention during the protein preparation process. Proposed strategy involving in silico gene design and automated cell-free expression and purification greatly reduces time and laboratorial efforts for protein preparation and thus would provide a versatile platform for the rapid and parallel materialization of sequence information.

키워드

저자

• Ho-Choel KIM [ Department of Fine Chemical Engineering and Applied Chemistry, Chungnam National University, Daejeon 305-764, Republic of Korea. ]
• Jin-Ho AHN [ Department of Fine Chemical Engineering and Applied Chemistry, Chungnam National University, Daejeon 305-764, Republic of Korea, Present address: Biochemicals Group 1, Samsung Petrochemical Corporation, Yongin-city, Gyeonggi-do 446-712, Republic of Korea. ]
• Jung-Won KEUM [ Department of Fine Chemical Engineering and Applied Chemistry, Chungnam National University, Daejeon 305-764, Republic of Korea, Present address: Department of Chemical Engineering, University of Massachusetts, Amherst, MA 01003, USA. ]
• Dong-Myung KIM [ Department of Fine Chemical Engineering and Applied Chemistry, Chungnam National University, Daejeon 305-764, Republic of Korea. ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  한국생물공학회 학술대회

• 간기

  반년간

• 수록기간

  1985~2013

• 십진분류

  KDC 476 DDC 576