Earticle

초록

영어

Solid freeform fabrication (SFF) is recognized as a promising tool for creating tissue engineering scaffolds due to advantages such as superior interconnectivity and highly porous structure. Despite structural support for SFF-based three-dimensional (3D) scaffolds that can lead to tissue regeneration, lack of cell recognition motifs and/or biochemical factors has been considered a limitation. Previously, recombinant mussel adhesive proteins (MAPs) were successfully demonstrated to be functional cell adhesion materials on various surfaces due to their peculiar adhesive properties. Herein, MAPs were applied as surface functionalization materials to SFF-based 3D polycaprolactone/poly(lactic-co-glycolic acid) scaffolds. We successfully coated MAPs onto scaffold surfaces by simply dipping the scaffolds into the MAP solution, which was confirmed through X-ray photoelectron spectroscopy and scanning electron microscopy analyses. Through in vitro study using human adipose tissue-derived stem cells (hADSCs), significant enhancement of cellular activities such as attachment, proliferation, and osteogenic differentiation was observed on MAP-coated 3D scaffolds, especially on which fused arginine–glycine–aspartic acid peptides were efficiently exposed. In addition, we found that in vivo hADSC implantation with MAP-coated scaffolds enhanced bone regeneration in a rat calvarial defect model. These results collectively demonstrate that facile surface functionalization of 3D scaffolds using MAP would be a promising strategy for successful tissue engineering applications.

키워드

저자

• Bum Jin KIM [ School of Interdisciplinary Bioscience and Bioengineering, Pohang University of Science and Technology, Pohang 790-784, Korea. ]
• Jung Min HONG [ Department of Mechanical Engineering, Pohang University of Science and Technology, Pohang 790-784, Korea. ]
• Kyung Shin KANG [ Department of Mechanical Engineering, Pohang University of Science and Technology, Pohang 790-784, Korea. ]
• Ki-Joo KIM [ Department of Plastic Surgery, Department of Molecular Biomedicine, College of Medicine, The Catholic University of Korea, Seoul 137-701, Korea. ]
• Jong Won RHIE [ Department of Plastic Surgery, College of Medicine, The Catholic University of Korea, Seoul 137-701, Korea. ]
• Dong-Woo CHO [ Department of Mechanical Engineering, Division of Integrative Biosciences and Biotechnology, Pohang University of Science and Technology, Pohang 790-784, Korea. ]
• Hyung Joon CHA [ School of Interdisciplinary Bioscience and Bioengineering, Department of Chemical Engineering, Pohang University of Science and Technology, Pohang 790-784, Korea. ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  한국생물공학회 학술대회

• 간기

  반년간

• 수록기간

  1985~2013

• 십진분류

  KDC 476 DDC 576