Inhibitory effect of Eisenia bicyclis extract on mRNA and protein expression of genes related in drug-resistance in methicillin-resistant Staphylococcus aureus cells
Jae-Hong PARK, Ji-Il CHOI, Yang-Bong LEE, Young-Mog KIM
언어
영어(ENG)
URL
https://www.earticle.net/Article/A174330
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원문정보
초록
영어
This study was performed to elucidate an antibacterial mechanism of Eisenia bicyclis extracts against methicillin-resistant Staphylococcus aureus (MRSA). Among of the extracts, the ethyl acetate soluble extract of E. bicyclis exhibited the highest anti-MRSA activity ranging in 128 μg/mL of minimum inhibitory concentration (MIC) against MRSA. Indeed, ethyl acetate extract was assessed its synergistic effects in combination with ß-lactam, ampicillin, penicillin and oxacillin against MRSA. Based on these results, we hypothesized that the ethyl acetate extraxct might inhibit a gene(s) or protein(s) related the drug resistance. It was observed that the ethyl acetate extract inhibited the mRNA expression of the resistant genes (mecI, mecR1 and mecA) in a dosedependent manner. Moreover, the results of Western blotting assays indicated that the ethyl acetate extract inhibited the synthesis of PBP2a protein. These results will provide a possible anti-MRSA mechanism by the ethyl acetate soluble extract of E. bicyclis.
키워드
antibacterialEisenia bicyclisMRSA
저자
Jae-Hong PARK [ Dept. of Food Science and Technology, Pukyong National University, Busan 608-737. ]
Ji-Il CHOI [ Dept. of Food Science and Technology, Pukyong National University, Busan 608-737. ]
Yang-Bong LEE [ Dept. of Food Science and Technology, Pukyong National University, Busan 608-737. ]
Young-Mog KIM [ Dept. of Food Science and Technology, Pukyong National University, Busan 608-737. ]
한국생물공학회 [The Korean Society for Biotechnology and Bioengineering]
설립연도
1984
분야
공학>생물공학
소개
이 법인은 생물 공학의 발전과 보급에 이바지하고, 회원 상호 간의 연구 협력과 친목을 도모함을 목적으로 한다
1. 생물공학 분야의 발전을 위한 연구 협력
2. 생물공학의 실용화를 촉진시키기 위한 산학 협동
3. 학술연구 발표회, 강연회, 연수회 등 학술활동의 개최
4. 국,영문 학술지,소식지,학술회의 Proceedings 및 학술도서의 발간
5. 생물공학 발전을 위한 정책 건의
6. 기타 국제 교류 등 본 학회의 목적 달성을 위한 제반 활동