Earticle

초록

영어

Recent biomimetic engineering has been driving toward a multifunctional cellular mimic system, which contain an agglomeration for all living metabolites: a mimicry of a nuclear pore transporter for selective mass transfer at the interface of nucleus and cytosol1,2; a virus-like assembly for effective drug delivery3; an artificial dendritic cell for tunable immune system.4 Inspired by these achievements, we sought to design a genenetworking hydrogel (G-net-gel), resembling an organized chromosome in the cell nucleus. It was spherodically manufactured using both simple lithography and DNA engineering methods. Stable transgene crossnetworks have been optimized in various parameters to accomplish enhanced in vitro protein expression efficiency. Furthermore, once with lipid capping, it completely reproduced the natural nucleus system, demonstrating the improved level of messenger RNAs relative to solution phase vectors. It is speculated that the proto-nucleus biomimetics would be a substitute to the conventional gene therapy, by which it overcome several barriers such as impaired transgene incorporations, and be a ground-breaking stratagem for gene-associated molecular therapeutics.

키워드

저자

• Sun Ju BAE [ Department of Chemical Engineering, Sungkyunkwan University, Suwon, Gyeonggi-do, 440-760. ]
• Kyung Ho NAM [ Department of Chemical Engineering, Sungkyunkwan University, Suwon, Gyeonggi-do, 440-760. ]
• Soong Ho UM [ Department of Chemical Engineering, Sungkyunkwan University, Suwon, Gyeonggi-do, 440-760. ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  한국생물공학회 학술대회

• 간기

  반년간

• 수록기간

  1985~2013

• 십진분류

  KDC 476 DDC 576