Earticle

초록

영어

ecent development of microbial platforms for the production of bisabolene, a precursor of bisabolane, biosynthetic alternative to D2 diesel fuel, has been stimulated due to rising petroleum costs and environmental concerns (1). Microbial engineering based on Synthetic biology boosts the efforts to produce ‘Drop-In’ biofuels from renewable feedstocks. Synthetic biology aims to develop the foundational understanding and technologies to build intelligent biological systems to accomplish the breakthrough especially of metabolic engineering. In this presentation, I will present the IPTG-free bisabolene production system using engineered E. coli. Quorum-sensing (QS) mediated E. coli enables to induce neighboring cells as well as itself to initiate the carbon flux to the designated metabolic pathway without using expensive Isopropyl-b-D-1- thiogalactopyranoside (IPTG), a widely-used inducer, and showed the similar capability of genetic controls compared to the IPTG-induced system. Several genetic modules were constructed to link QS-system and biofuel pathway. Direct QS-system depending on cell density led the same level of bisabolene productions in E. coli. This QS-system for biofuels production could be a cost-effective and promising strategy to develop microbial platforms by reducing the usage of the exogenous inducer. (1) Peralta-Yahya et al., 2011 Nat. Commun.

키워드

저자

• Han Min WOO [ Clean Energy Research Center, Korea Institute of Science and Technology, Seoul, Republic of Korea. ]
• Jay KEASLING [ Joint BioEnergy Institute, Physical Bioscience Division, Lawrence Berkeley National Laboratory, Emeryville, California, 94608 USA. ]
• Taek Soon LEE [ Joint BioEnergy Institute, Physical Bioscience Division, Lawrence Berkeley National Laboratory, Emeryville, California, 94608 USA ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  한국생물공학회 학술대회

• 간기

  반년간

• 수록기간

  1985~2013

• 십진분류

  KDC 476 DDC 576