An integrated allele-specific (AS) polymerase chain reaction (PCR) and resultant amplicon detection system has been developed for multiplex single nucleotide polymorphism (SNP) genotyping on a portable instrumentation, which was applied for on-site identification of HANWOO. As for the detection method, micro-capillary electrophoresis (CE) and microarray were employed, and the integrated PCR-μCE and PCR-microarray devices were constructed. Twelve sets of primers were designed for targeting eleven SNP loci and one primer set for a positive PCR control. The operation of microdevices were automated with a portable genetic analyzer, and the detection was performed on a miniaturized fluorescence detector for CE separation or a fluorescence scanner for microarray. Successful HANWOO verification was conducted with 99.37% fidelity in 90 min. Thus, our genetic analysis system provides rapid, accurate, and on-site multiplex SNP typing platform.
Jong Young CHOI [ Department of Chemical and Biomolecular Engineering (BK21 Program) and KAIST Institute for the BioCentury, Korea Advanced Institute for Science and Technology (KAIST), Daejeon, 305-701. ]
Tae Seok SEO [ Department of Chemical and Biomolecular Engineering (BK21 Program) and KAIST Institute for the BioCentury, Korea Advanced Institute for Science and Technology (KAIST), Daejeon, 305-701. ]
한국생물공학회 [The Korean Society for Biotechnology and Bioengineering]
설립연도
1984
분야
공학>생물공학
소개
이 법인은 생물 공학의 발전과 보급에 이바지하고, 회원 상호 간의 연구 협력과 친목을 도모함을 목적으로 한다
1. 생물공학 분야의 발전을 위한 연구 협력
2. 생물공학의 실용화를 촉진시키기 위한 산학 협동
3. 학술연구 발표회, 강연회, 연수회 등 학술활동의 개최
4. 국,영문 학술지,소식지,학술회의 Proceedings 및 학술도서의 발간
5. 생물공학 발전을 위한 정책 건의
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