Earticle

초록

영어

In vitro techniques are efficient tools for evaluating the effects of newly developed chemicals, drugs and nanomaterials on human beings. We have recently developed a cell-based chip that enables the sensitive detection of cell viability using electrochemical methods. Peptide nanopatterned array was fabricated on the electrode surface as nanodots, nanorods or nanopillar structures to enhance cell adhesion, spreading and proliferation. The enhanced cell adhesion on the electrode surface accelerates the electron transfer rate at the cell-electrode interfaces, which increases the sensitivity of fabricated cell chip. The redox signals obtained from neural cells via voltammetric tools were proportional to the cell viability which was used for assessing the toxicity of environmental toxins, as well as for evaluating the efficiency of anticancer drugs. The nanofabrication technology also enabled the integration of a powerful analysis tool, Surface-enhanced Raman spectroscopy (SERS), which can investigate the changes of intracellular composition and the effects of anticancer drugs on single cancer cells precisely. Proposed cell chip based on nanofabrication technology can be applied as an invitro analysistoolinvariouskindsofneural cell researches.

저자

• Jeong-Woo CHOI [ Department of Chemical & Biomolecular Engineering and Graduate School of Management of Technology, Sogang University, Seoul 121-742, Korea ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  한국생물공학회 학술대회

• 간기

  반년간

• 수록기간

  1985~2013

• 십진분류

  KDC 476 DDC 576