Earticle

초록

영어

Induced conversion of cell types and targeted genetic modification have been challenging in mammalian cells including human cells. It has been recently shown that one specific cell type can be converted into another through epigenetic modification using only a handful of reprogramming factors. In addition, significant breakthroughs have been made in targeted genetic modification. We will first briefly review the recent progress in the cell fate conversions including the reprogramming of somatic cells into pluripotent stem cells and direct lineage change between differentiated cells. This induced cell type conversion is thought to occur through epigenetic modification. We will next present recent progresses on the targeted genetic modification of mammalian cells using zinc finger nucleases, especially combined with induced cell fate conversion. We have developed a surrogate reporter system which enables efficient enrichment of cells of which genomes have been edited using zinc finger nucleases. These targeted engineering of mammalian cells through epigenetic and genetic modification will greatly contribute both to the establishment of research models for human diseases and to the development of therapeutic modality for these diseases.

저자

• Hyongbum KIM [ Graduate School of Biomedical Science and Engineering, Hanyang University, Seoul, South Korea ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  한국생물공학회 학술대회

• 간기

  반년간

• 수록기간

  1985~2013

• 십진분류

  KDC 476 DDC 576