Earticle

초록

영어

Anaplerotic carboxylation refers the linkage reactions between glycolysis and TCA cycle mediating carboxylation of C3 metabolites into C4 metabolites. The energy for carbon-carbon bondage formation derives from difference sources in the anaplerotic enzymes, and the artificial anaplerotic carboxylation was affected by intracellular modulating metabolite concentrations, cofactors(3), and the way of regulations(2). Artificial expression of anaplerotic enzymes such as phosphoenolpyruvate carboxylase(PPC), phosphoenolpyruvate carboxykinase(PCK), and NADP-dependent malic enzyme(MaeB) enhanced the carboxylation of C3 metabolites under anaerobic with surplus of CO2 source (119 mM bicarbonate) conditions(4). The availability of CO2 source for the anaplerotic enzymes were corelated to the C4 metabolite production(5). The NAD-dependent Malic enzyme(MaeA) expression mediated decarboxylation under the same conditions. Cellular energy currencies related to the anaplerotic enzyme expressions were modified depending on their chemical energy source for carboxylation, i.e., PEP for PPC, PEP and ATP for PCK, NADPH for MaeB, and NADH for MaeA, and on their modulating metabolite concentrations. The examples of cellular energy currency modifications are introduced as well as the cellular responses therefrom(1, 6).

키워드

저자

• Pil KIM [ Dept. of Biotechnology, Catholic University of Korea, Bucheon, Gyeonggi 420-743, Korea. ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  한국생물공학회 학술대회

• 간기

  반년간

• 수록기간

  1985~2013

• 십진분류

  KDC 476 DDC 576