Earticle

초록

영어

The carbon dioxide (CO2) emissions are main cause for global climate change. It becomes critical to develop novel technologies that can subside the problem. The biotechnology-based CO2 capture tool is one of the important and potential systems for solving the CO2-revoked climate problems. Carbonic anhydrase (CA, EC 4.2.1) accelerates the uptake of CO2 from air into intracellular system. Recently, we cloned several types of CAs from marine microalgae or bacteria and we constructed their sequences optimized for high-efficient expression in E. coli system. We achieved successful microbial expression of several CAs and characterized the properties of the recombinant CAs. The acquired CAs can mediate biomineralization by catalyzing the dissolved CO2 to bicarbonate (HCO3 -), which can be precipitated as CaCO3 under Ca2+ condition. In addition, HCO3 - concentrated by CAs can be converted with PEP (phosphoenolpyruvate) into four-carbon organic acid oxaloacetate (OAA) catalyzed by phosphoenolpyruvate carboxylase (PEPCase, EC 4.1.1.31). These two enzyme system can be used as novel platform technology for CO2 capture and use. These biotechnologybased approaches based on marine CA will be essentially employed for development of efficient CO2 capture/sequestration systems.

키워드

저자

• Seung Pil PACK [ Dept. of Biotechnology and Bioinformatics, Korea University, Chungnam, 339-700. ]
• Bashistha KANTH [ Dept. of Biotechnology and Bioinformatics, Korea University, Chungnam, 339-700. ]
• Ki Ha MIN [ Dept. of Biotechnology and Bioinformatics, Korea University, Chungnam, 339-700. ]
• Mi Ran KI [ Dept. of Biotechnology and Bioinformatics, Korea University, Chungnam, 339-700. ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  한국생물공학회 학술대회

• 간기

  반년간

• 수록기간

  1985~2013

• 십진분류

  KDC 476 DDC 576