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돼지 미성숙 난포란의 체외성숙에 미치는 Thymidine처리 효과
Effect of Thymidine on In Vitro Maturation of Immature Porcine Follicular Oocytes
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- 발행기관
- 한국동물생명공학회(구 한국동물번식학회) 바로가기
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- 간행물
- Reproductive & developmental biology 바로가기
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- 통권
- Volume 36 No 1 (2012.03)바로가기
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- 페이지
- pp.71-77
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- 저자
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- 언어
- 한국어(KOR)
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- URL
- https://www.earticle.net/Article/A172416
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원문정보
초록
- 영어
- The objective of this study was to examine the effect of thymidine treatment during in vitro maturation (IVM) of porcine follicular oocytes on blastocyst development. Porcine oocytes were treated with thymidine (10 mM, 20 mM and 30 mM) for 2 or 6 hr in the preiods of IVM I and/or II. The survival rates of the blastocysts in the 6 hr treatment groups of 10 mM and 20 mM during IVM I period were significantly higher than those of control group (p<0.05). However, the survival rate of the blastocysts in the 2 hr treatment group of 20 mM during IVM II period was significantly higher than control group (p<0.05). Furthermore, the survival rate of the blastocysts in the 6 hr treatment group of 30 mM during IVM II period was significantly lower than control group (p<0.05). Consistent with the previous result, blastocyst development of both IVM I and II treatment group was also showed as similar pattern. Total and apoptotic cell numbers of blastocysts derived from thymidine treated porcine oocytes were examined by using Tunel assay. The results showed that there was no significant differences in total cell number of blastocysts between thymidine treated and untreated groups. However, apoptosis-positive cells in the thymidine treated group (6 hr IVM I) were significantly lower than those of other groups (p<0.05). Taken together, these results indicate that high quality oocytes were selected by DNA synthesis mechanism according to high concentration thymidine treatment during porcine oocyte maturation. Therefore, we concluded that presumptive selected oocytes by thymidine treatment during maturation periods improved the further embryo development and embryonic quality of IVF embryos by decreasing the incidence of apoptosis in preimplantation porcine embryos.
목차
ABSTRACT
서론
재료 및 방법
배양액
체외 성숙, 수정 및 배양
TUNEL Assay
실험 설계
결과
체외성숙 기간 동안 Thymidine 처리 효과
Thymidine 처리에 의해 체외생산된 배반포의 질적 분석
고찰
인용문헌
키워드
IVM Thymidine Tunel assay Embryonic selection Pig저자
간행물 정보
발행기관
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- 발행기관명
- 한국동물생명공학회(구 한국동물번식학회) [The Korean Society of Animal Reproduction and Biotechnology]
- 설립연도
- 1976
- 분야
- 농수해양>축산학
- 소개
- 동물번식생리학, 동물생명공학, 수의학, 인공수정 및 수정란이식을 이용한 동물개량에 관한 이론과 기술의 발전을 통해 학계, 연구계, 산업계 및 양축가 상호간의 협력을 도모함으로써 동물과학발전 및 사회일반의 이익에 기여 한다는 목적을 위해 노력해 나가겠습니다.
간행물
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- 간행물명
- Reproductive & developmental biology
- 간기
- 계간
- pISSN
- 1738-2432
- eISSN
- 2288-0151
- 수록기간
- 1977~2018
- 십진분류
- KDC 527 DDC 636
이 권호 내 다른 논문 / Reproductive & developmental biology Volume 36 No 1
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