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Analysis of Sexed Sperm by Flow Cytometry in Hanwoo (Korean Native Cattle)

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  • 발행기관
    한국동물생명공학회(구 한국동물번식학회) 바로가기
  • 간행물
    Reproductive & developmental biology 바로가기
  • 통권
    Volume 36 No 1 (2012.03)바로가기
  • 페이지
    pp.1-6
  • 저자
    Han-Jun Yoo, Kyung-Jin Lee, Yong-Seung Lee, Pil-Sang Yoon, Joung-Jun Park, Hyeong-Cheol Kim, Choon-Keun Park
  • 언어
    영어(ENG)
  • URL
    https://www.earticle.net/Article/A172373

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원문정보

초록

영어
This study evaluated a sexed sperm ability to produce embryos by flow cytometer. Hanwoo bulls sperm were separated to X and Y sperm via Hoechst 33342 stained with near UV laser or performed the pre-sorted without near UV laser beam in flow cytometry. Pre-sorted sperm had significantly higher viability (84±1.15 %, p<0.05) compared to other sorted groups in frozen-thawed semen. For fresh semen, pre-sorted sperm had the higher viability (79±3 %, p<0.05) than those of the X and Y sperm (44.7±1.67 and 41.7±1.2 %) separated by differences of DNA content. On the other hand, pre-sorted and X sperm sorted according to differences in DNA content had significantly higher viabilities (24.3±1.2 and 25.7±0.9 %, p<0.05) compared to that of the sorted Y sperm (13.7±1.2 %) in the hypoosmotic swelling test. The proportion acrosome reaction in the sorted X sperm was higher (55.0±1.7 and 45.0±1.5 %) than those of the sorted Y-sperm (32.3±0.9 %, p<0.05). However, the sperm morphologies of the sorted groups were not significantly differences. In conclusion, the sex-sorting procedure by flow cytometry affected some characteristics of Hanwoo sperm. Further study is needed to determine the optimal procedures to enhance male and female embryos and sorting accuracy.

목차

ABSTRACT
 INTRODUCTION
 MATERIALS AND METHODS
  Preparation of Sheath Fluid Buffer
  Cryopreservation and Thawing of Sperm
  Sperm Pretreatment
  Hoechst 33342 Staining
  Sex-Sorting of Sperm
  Sperm Analysis
  Statistical Analysis
 RESULTS
 DISCUSSTION
 REFERENCES

키워드

Flow cytometry Sperm sorting Sperm analysis Hanwoo)

저자

  • Han-Jun Yoo [ Developmental Biotechnology Laboratory, Myung-poom Hanwoo Consulting, Hoengseong 225-807, Korea ]
  • Kyung-Jin Lee [ College of Animal Life Sciences, Kangwon National University, Chuncheon 200-701, Korea ]
  • Yong-Seung Lee [ College of Animal Life Sciences, Kangwon National University, Chuncheon 200-701, Korea ]
  • Pil-Sang Yoon [ Developmental Biotechnology Laboratory, Myung-poom Hanwoo Consulting, Hoengseong 225-807, Korea ]
  • Joung-Jun Park [ Developmental Biotechnology Laboratory, Myung-poom Hanwoo Consulting, Hoengseong 225-807, Korea ]
  • Hyeong-Cheol Kim [ Hanwoo Experiment Station, National Livestock Research Institute, Pyeongchang 232-952, Korea ]
  • Choon-Keun Park [ College of Animal Life Sciences, Kangwon National University, Chuncheon 200-701, Korea ] Corresponding author

참고문헌

자료제공 : 네이버학술정보

간행물 정보

발행기관

  • 발행기관명
    한국동물생명공학회(구 한국동물번식학회) [The Korean Society of Animal Reproduction and Biotechnology]
  • 설립연도
    1976
  • 분야
    농수해양>축산학
  • 소개
    동물번식생리학, 동물생명공학, 수의학, 인공수정 및 수정란이식을 이용한 동물개량에 관한 이론과 기술의 발전을 통해 학계, 연구계, 산업계 및 양축가 상호간의 협력을 도모함으로써 동물과학발전 및 사회일반의 이익에 기여 한다는 목적을 위해 노력해 나가겠습니다.

간행물

  • 간행물명
    Reproductive & developmental biology
  • 간기
    계간
  • pISSN
    1738-2432
  • eISSN
    2288-0151
  • 수록기간
    1977~2018
  • 십진분류
    KDC 527 DDC 636

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