Earticle

초록

영어

In the screening process of organic solvent tolerant bacteria showing good growth in media containing several kinds of organic solvents, one strain was isolated and identified as Bacillus sp. BCNU 5006. The strain was able to tolerate many organic solvents including benzene, toluene, xylene, octane, dodecane, butanol and ethylbenzene. Likewise, it could also utilize these solvents as the sole source of carbon with significant enzyme production. The lipolytic enzyme stability of Bacillus sp. BCNU 5006 was studied in the presence of several kinds of solvents at a 25% (v/v) concentration. The highest enzyme stability was observed in the presence of octane (107%), followed by ethylbenzene (88%), decane (86%), and chloroform (85%). Especially, BCNU 5006 lipase was determined to be more stable than immobilized enzyme (Novozyme 435) in the presence of octane, chloroform and xylene. This organic solvent tolerant Bacillus sp. BCNU 5006 could be expected as a potential bioremediation agent and biocatalyst for biodegradation and provide on organicsolvent-based enzymatic synthetic method in industrial chemical processes.

목차

Abstract
 1. 서론
 2. 재료 및 방법
  2.1. 유기용매 내성 균주 분리
  2.2. 분리균주의 생리 ․ 생화학적 특성 및 동정
  2.3. 유기용매 내성 및 분해능 조사
  2.4. Lipase 생서능 조사 및 조효소액 조제
  2.5. Lipase 활성도 측정
  2.6. 효소의 온도 및 pH 안정성 조사
  2.7. 다양한 유기용매에 대한 효소의 안정성 조사
  2.8. 금속이온의 효소활성에 미치는 영향 조사
 3. 결과 및 고찰
  3.1. 유기용매 내성 세균 분리
  3.2. 생리 ․ 생화학적 특성 및 동정
  3.3. Bacillus sp. BCNU 5006의 유기용매 내성 및 분해능
  3.4. 유기용매 내성 세균이 생산하는 lipase의 다양한 온도와 pH에서의 lipase 활성
  3.5. Lipase의 안정성 및 활성에 미치는 유기용매의 영향
  3.6. Lipase 활성에 미치는 금속이온의 영향
 4. 결론
 References

키워드

저자

• 최혜정 [ Hye Jung Choi | 창원대학교 생물공학협동과정 ]
• 황민정 [ Min Jung Hwang | 창원대학교 생물학과 ]
• 김봉수 [ Bong Su Kim | 창원대학교 생물학과 ]
• 정영기 [ Yong-Kee Jeong | 동아대학교 생명공학과 ]
• 주우홍 [ Woo Hong Joo | 창원대학교 생물공학협동과정, 2창원대학교 생물학과 ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  KSBB Journal

• 간기

  격월간

• pISSN

  1225-7117

• 수록기간

  2009~2013

• 십진분류

  KDC 476 DDC 576