Earticle

초록

영어

Substantial efforts have been made to manipulate ruminal environment in a hope to enhance ruminal fermentation efficiency for better ruminant productivity. Some of examples are methane inhibitors, antibiotics, microbial enzymes, fatty acids and/or lipid feeding, buffering agents, ionophores and probiotics. Of these efforts, the non-ionic surfactant (NIS) has been known for its stimulation to release enzymes from a range of anaerobic microbes. A series of studies were conducted 1) to evaluate NIS diluted with water and ethanol on in vitro ruminal fermentation and 2) to determine the influence of diluted NIS on digestibility of feedstuffs. In first experiment (Exp. 1), NIS was diluted with water or ethanol to measure its effects on in vitro microbial growth, ruminal enzyme activities and gas production by mixed ruminal microbial culture. The NIS was diluted with water or ethanol separately in a 1:5 ratio (w/v). Water and ethanol-diluted NIS with wheat flour were added with rice straw-based mixed ruminal microbial cultures at the rate of 2 ㎎ NIS/16 ㎖ McDougall buffer plus 4 ml ruminal fluid solution. The mixed ruminal microbial culture was run without any NIS addition as control. Addition of NIS either diluted with water or ethanol has significantly reduced the gas production in mixed ruminal microbial culture at 12 and 24 h of incubation. At 48 h post incubation, gas production was the highest with the addition of ethanol diluted NIS followed by water-diluted NIS and control. Carboxy methyl cellulase activity in rice straw-based mixed ruminal bacterial culture was significantly higher with the addition of ethanol-diluted NIS compared with the addition of water-diluted NIS and control at 24 and 72 h post incubation. In second in vitro experiment (Exp. 2), effects of addition of ethanol diluted NIS on dry matter (DM) digestibility of alfalfa hay, gas production, pH and cellular growth in mixed ruminal microbial culture were examined. Alfalfa hay based mixed ruminal microbial culture without any NIS addition was run as a control. The pH of mixed ruminal microbial culture was significantly lower than control at all post incubation sampling hours. In vitro DM digestibility of alfalfa hay was significantly higher with the addition of NIS compared with control. Gas production was significantly less with NIS addition compared with control at all post incubation sampling hours. Microbial growth in mixed ruminal microbial culture was significantly increased with the addition of NIS compared to control.

목차

ABSTRACT
 I. 서론
 II. 재료 및 방법
  1. 공시동물 및 공시기질
  2. 시험설계
  3. 조사항목 및 측정방법
  4. 통계분석
 III. 결과 및 고찰
  시험 1.
   1. 가스 생성량
   2. CMCase와 xylanase의 활력
  시험 2
   1. In vitro pH
   2. In vitro 건물 소화율
   3. Gas 생성량 및 미생물의 성장
 IV. 요약
 V. 인용문헌

키워드

저자

• 이왕식 [ Wang Shik Lee | 농촌진흥청 국립축산과학원 ] Corresponding Author
• 김현섭 [ Hyeon Shup Kim | 농촌진흥청 국립축산과학원 ]
• 기광석 [ Kwang Seok Ki | 농촌진흥청 국립축산과학원 ]
• 이현준 [ Hyun June Lee | 농촌진흥청 국립축산과학원 ]
• 김상범 [ Sang Bum Kim | 농촌진흥청 국립축산과학원 ]
• 백광수 [ Kwang Soo Baek | 농촌진흥청 국립축산과학원 ]
• 홍성구 [ Seong Koo Hong | 농촌진흥청 국립축산과학원 ]
• 김상호 [ Sang Ho Kim | 농촌진흥청 국립축산과학원 ]
• 박준철 [ Jun Cheol Park | 농촌진흥청 국립축산과학원 ]
• 조영무 [ Young Moo Cho | 농촌진흥청 국립축산과학원 ]
• 이상철 [ Sang Cheol Lee | 농촌진흥청 국립축산과학원 ]
• 하종규 [ Jong Kyu Ha | 서울대학교 농생명공학부 ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  동물자원연구 [Annals of Animal Resources Sciences]

• 간기

  계간

• pISSN

  1225-2964

• eISSN

  2287-3317

• 수록기간

  1999~2026

• 등재여부

  KCI 등재

• 십진분류

  KDC 527 DDC 636