Earticle

초록

영어

Whey based medium was optimized for the production of viable Lactobacillus crispatus KLB 46 isolated from the vagina of Korean women. Among the various nitrogen sources such as yeast extract, beef extract, and proteose peptone no. 3 supplemented to whey, beef extract showed the highest viable cell production. The addition of Tween 80 to the whey based medium increased viable cell concentration. As beef extract supplementation is not economically attractive, corn steep liquor was added as a supplementary nitrogen sources. When corn steep liquor was supplied with beef extract with the ratio 5：1, the viable cell count was 3.11 × 109 CFU/mL. Also, the addition of mineral salts containing sodium acetate (5 g/L), potassium phosphate dibasic (2 g/L), magnesium sulfate (0.1 g/L) and manganese sulfate (0.05 g/L) to the whey medium increased viable cell count further (5.00 × 109 CFU/mL).

목차

Abstract
 1. 서론
 2. 재료 및 방법
  2.1. Strain, medium and culture condition
  2.2. Aerobic and anaerobic cultures
  2.3. Whey-based medium
  2.4. Analytical methods
 3. 결과 및 고찰
  3.1. Aerobic and anaerobic cultures
  3.2. Effect of nitrogen source
  3.3. Effect of Tween 80
  3.4. Use of CSL as nitrogen source
  3.5. Effect of trace element
 4. 결론
 References

키워드

저자

• 장정은 [ Chung Eun Chang | 인하대학교 생명화학공학부 ]
• 구자룡 [ Ja-Ryong Koo | 인하대학교 생명화학공학부 ]
• 소재성 [ Jae-Seong So | 인하대학교 생명화학공학부 ]
• 윤현식 [ Hyun Shik Yun | 인하대학교 생명화학공학부 ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  KSBB Journal

• 간기

  격월간

• pISSN

  1225-7117

• 수록기간

  2009~2013

• 십진분류

  KDC 476 DDC 576