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Toxicity and Biomedical Imaging of Fluorescence-Conjugated Nanoparticles in Hematopoietic Progenitor Cells
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- 발행기관
- 한국동물생명공학회(구 한국동물번식학회) 바로가기
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- 간행물
- Reproductive & developmental biology 바로가기
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- 통권
- Volume 35 No 4 (2011.12)바로가기
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- 페이지
- pp.503-510
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- 저자
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- 언어
- 영어(ENG)
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- URL
- https://www.earticle.net/Article/A163589
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초록
- 영어
- Cellular uptake of nanoparticles for stem cell labeling and tracking is a critical technique for biomedical therapeutic applications. However, current techniques suffer from low intracellular labeling efficiency and cytotoxic effects, which has led to great interest in the development of a new labeling strategy. Using silica-coated nanoparticles conjugated with rhodamine B isothiocyanate (RITC) (SR), we tested the cellular uptake efficiency, biocompatibility, proliferation or differentiation ability with murine bone marrow derived hematopoietic stem/progenitor cells. The bone marrow hematopoietic cells showed efficient uptake with SR with dose or time dependent manner and also provided a higher uptake on hematopoietic stem/progenitor cells. Biocompatibility tests revealed that the SR had no deleterious effects on cell cytotoxicity, proliferation, or multi-differentiation capacities in vitro and in vivo. SR nanoparticles are advantageous over traditional labeling techniques as they possess a high level of cellular internalization without limiting the biofunctionality of the cells. Therefore, SR provides a useful alternative for gene or drug delivery into hematopoietic stem/progenitor cells for basic research and clinical applications.
목차
ABSTRACT
INTRODUCTION
MATERIALS AND METHODS
Preparation of Nanoparticles
Preparation of Hematopoietic Cells
Flow Cytometry Analysis
Cell Viability and Proliferation Assay
Cell Cycle Analysis
Colony-Forming-Cell Ability Assay
In Vivo Hematopoietic Reconstitution Activity
Statistical Analysis
RESULTS
Uptake Efficiency into Bone Marrow Hematopoietic Cells
Cell Proliferation and Cytotoxic Effects
In Vitro Hematopoietic Differential Effects
In Vivo Hematopoietic Reconstitution Effects
Intracellular Uptake into Hematopoietic Stem/Progenitor Cells
DISCUSSION
REFERENCES
키워드
Hematopoietic stem/progenitor cells Silica nanoparticles Biocompatibility In vivo transplantation저자
간행물 정보
발행기관
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- 발행기관명
- 한국동물생명공학회(구 한국동물번식학회) [The Korean Society of Animal Reproduction and Biotechnology]
- 설립연도
- 1976
- 분야
- 농수해양>축산학
- 소개
- 동물번식생리학, 동물생명공학, 수의학, 인공수정 및 수정란이식을 이용한 동물개량에 관한 이론과 기술의 발전을 통해 학계, 연구계, 산업계 및 양축가 상호간의 협력을 도모함으로써 동물과학발전 및 사회일반의 이익에 기여 한다는 목적을 위해 노력해 나가겠습니다.
간행물
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- 간행물명
- Reproductive & developmental biology
- 간기
- 계간
- pISSN
- 1738-2432
- eISSN
- 2288-0151
- 수록기간
- 1977~2018
- 십진분류
- KDC 527 DDC 636
이 권호 내 다른 논문 / Reproductive & developmental biology Volume 35 No 4
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