Earticle

초록

영어

During mammalian fertilization, germ cell-specific hyaluronidases, such as sperm adhesion molecule 1 (SPAM1) and hyaluronoglucosaminidase 5 (Hyal5), are important for the dispersal of the cumulus mass. In this study, we demonstrated that bull Hyal5 is a single copy gene on chromosome 4 that is expressed specifically in the testis. In addition, we expressed recombinant bull SPAM1 and Hyal5 in human embryonic kidney 293T cells and showed that these enzymes possessed hyaluronidase activity. We also demonstrated that a polyclonal antibody against bull sperm hyaluronidase inhibits sperm-egg interactions in an in vitro fertilization (IVF) assay. Our results suggested that bull Hyal5 may have a critical role in bull fertilization.

목차

ABSTRACT
 INTRODUCTION
 MATERIALS AND METHODS
  Total RNA Extraction and Reverse Transcriptase-Polymerase Chain Reaction (RT-PCR)
  Southern Blot Analysis
  Antibody
  Preparation of Protein Extracts
  Sodium Dodecyl Sulfate-polyacrylamide Gel Electrophoresis(SDS-PAGE) and Western Blot Analysis
  Expression of Bovine PH-20 and Hyal5
  Zymography
  Dispersal of Cumulus Cells In Vitro
  In Vitro Maturation (IVM) and In Vitro Fertilization(IVF)
  Statistical Analysis
 RESULTS
  Expression of PH-20 and Hyal5
  Sequence Analysis of Hyal5 and SPAM1
  SPAM1 and Hyal5 are Single Copy Genes in the Bovine Genome
  Importance of Sperm Hyaluronidase in Sperm-Oocyte Interaction
 DISCUSSION
 REFERENCES

키워드

저자

• Ekyune Kim [ College of Pharmacy, Catholic University of Daegu ]
• Kyu-Tae Chang [ National Primate Research Center, Korea Research Institute of Bioscience and Biotechnology ] Corresponding author

참고문헌

발행기관

간행물

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• 간행물명

  Reproductive & developmental biology

• 간기

  계간

• pISSN

  1738-2432

• eISSN

  2288-0151

• 수록기간

  1977~2018

• 십진분류

  KDC 527 DDC 636