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Effects of Escherichia coli Contamination on Extended Porcine Semen Parameters

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  • 발행기관
    한국동물생명공학회(구 한국동물번식학회) 바로가기
  • 간행물
    Reproductive & developmental biology 바로가기
  • 통권
    Volume 35 No 4 (2011.12)바로가기
  • 페이지
    pp.479-483
  • 저자
    Kyoung Min So, Soo Jin Sa, Hyo Jin Kim, Ki Hwa Chung, Byeong Yeal Jung, Jung Ho Son, In Cheul Kim
  • 언어
    영어(ENG)
  • URL
    https://www.earticle.net/Article/A163585

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초록

영어
The objective of this study was to determine the effects of E. coli isolated from porcine semen on sperm viability, motility, and semen pH. Semen samples were prepared using commercial extender, SeminarkPro (Noahbio Tech, Korea) that did not contain antibiotics. And 4 different levels of E. coli were artificially innoculated to semen with following concentrations; 4,000 of sperms with 1 of E. coli (T1), 400 with 1 (T2), 40 with 1 (T3), and 4 with 1 (T4). Semen samples were preserved at 17℃ for 5 days in semen storage box until analyzed by flowcytometer. Aliquots were subjected to measure the sperm viability (Live/Dead® stain), motility (mitochondrial function), and semen acidity (pH) from day 0 (day of semen collection) to day 5. Sperm motility and viability were significantly decreased (p<0.05) on day 0 (4 hrs after preservation at 17℃) in T3 and T4 compared to control groups and were significantly decreased (p<0.05) in all groups from day 3. Sample pH was acidic in T3 (6.90~6.86) and T4 (6.86~6.65) from day 3 to day 5 (p<0.05).
On the other hand, sample pH was maintained 7.0~7.1 in control, T1, and T2 during the experimental period. Sperm motility and viability were significantly decreased from day 0 to day 5 compared to control in samples contaminated with E. coli above a value of 40:1 (20×106 sperm cells/ml : 5×105 cfu/ml). Even on day 1 in T4 and on day 3 in T3, semen pH was acidic probably due to the acidification of dead spermatozoa. These results suggest that E. coli contamination has a concentration-dependent detrimental effect on extended porcine semen quality.

목차

ABSTRACT
 INTRODUCTION
 MATERIALS AND METHODS
  Animals
  Semen Preparation
  Bacterial Isolation
  Analysis of Sperm Parameters
  Statistical analysis
 RESULTS
  Sperm Motility, Viability and Evaluation of Sperm Agglutination
  Semen pH
 DISCUSSION
 REFERENCES

키워드

Extended porcine semen E. coli Semen quality Flowcytometery Semen parameters

저자

  • Kyoung Min So [ National Institute of Animal Science, RDA ]
  • Soo Jin Sa [ National Institute of Animal Science, RDA ]
  • Hyo Jin Kim [ National Institute of Animal Science, RDA ]
  • Ki Hwa Chung [ Gyeongnam National University of Science and Technology ]
  • Byeong Yeal Jung [ Animal Disease Diagnostic Division, Animal, Plant and Fisheries Quarantine & Inspection Agency ]
  • Jung Ho Son [ Noah Biotech. Inc. ]
  • In Cheul Kim [ National Institute of Animal Science, RDA ] Corresponding author

참고문헌

자료제공 : 네이버학술정보

간행물 정보

발행기관

  • 발행기관명
    한국동물생명공학회(구 한국동물번식학회) [The Korean Society of Animal Reproduction and Biotechnology]
  • 설립연도
    1976
  • 분야
    농수해양>축산학
  • 소개
    동물번식생리학, 동물생명공학, 수의학, 인공수정 및 수정란이식을 이용한 동물개량에 관한 이론과 기술의 발전을 통해 학계, 연구계, 산업계 및 양축가 상호간의 협력을 도모함으로써 동물과학발전 및 사회일반의 이익에 기여 한다는 목적을 위해 노력해 나가겠습니다.

간행물

  • 간행물명
    Reproductive & developmental biology
  • 간기
    계간
  • pISSN
    1738-2432
  • eISSN
    2288-0151
  • 수록기간
    1977~2018
  • 십진분류
    KDC 527 DDC 636

이 권호 내 다른 논문 / Reproductive & developmental biology Volume 35 No 4

    피인용수 : 0(자료제공 : 네이버학술정보)

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