Ji-Hoon Park, Young-Ho Chung, Man-Hee Rhee, Sang-Keun Kim
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영어(ENG)
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https://www.earticle.net/Article/A163584
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영어
In the present study, the effect of cysteine and NT or bisphenol A (BP) on in vitro aturation (IVM) of porcine oocytes were examined. COCs was cultured in NCSU-23 medium supplement with 10% FCS which had previously been covered with mineral oil and equilibrated in a humidified atmosphere of 5% CO2 and 95% air at 38℃. The IVM rate of oocytes cultured for 48 hrs in NCSU-23 medium supplement with 0.5~10.0 mM cysteine were 34.0±3.2%, 36.0±3.5%, 48.0±3.8%, 22.0±3.2%, respectively. The IVM rate of oocytes cultured in NCSU-23 medium supplement with 0.5~5.0 mM NT for 48 hrs were 24.0±4.2%, 18.0±4.9%, 8.0±2.2%, respectively. NT affects oocyte in vitro maturation rate in a dose-dependent. This result were significantly lower than the control group. The IVM rate of oocytes cultured for 48 hrs in NCSU-23 medium supplement with 1.0 mM NT+5.0 mM cysteine (38.0±4.3%) were significantly higher than that of NT treatment. The IVM rate of oocytes cultured in NCSU-23 medium supplement with 0.05~5.0 mM BP for 48 hrs were 20.0±4.7%, 10.0±5.3%, 6.0±3.2%, respectively. The IVM rate of oocytes cultured in NCSU-23 medium supplement with BP was significantly lower cultured non supplement of BP (44.0±3.5%). BP affects porcine oocyte maturation rate in a dose-dependent manner. The IVM rate of oocytes cultured for 48 hrs in NCSU-23 medium supplement with 1.0 mM BP+5.0 mM cysteine (32.0±3.2%) were increased than that of BP treatment.
목차
ABSTRACT INTRODUCTION MATERIALS AND METHODS In Vitro Maturation of Oocytes Supplementation with Ginsenoside, NT and BP Assessment of Meiotic Stage Statistical Analysis RESULTS AND DISCUSSION Effect of Cysteine Concentration on IVM Rate Effect of Cysteine to NT on IVM Rate Effect of Cysteine to BP on IVM Rate REFERENCES
키워드
CysteineNTBPIVMPorcine oocyteToxic reduction
저자
Ji-Hoon Park [ College of Veterinary Medicine, Chungnam National University ]
Young-Ho Chung [ Dept. of Companion Animal and Animal Resourses Science, Joongbu University ]
Man-Hee Rhee [ College of Veterinary Medicine, Kyungpook National University ]
Sang-Keun Kim [ College of Veterinary Medicine, Chungnam National University ]
Corresponding author