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Changes in Plasminogen Activity in Uterus Tissue during

첫 페이지 보기
  • 발행기관
    한국동물생명공학회(구 한국동물번식학회) 바로가기
  • 간행물
    Reproductive & developmental biology 바로가기
  • 통권
    Volume 35 No 4 (2011.12)바로가기
  • 페이지
    pp.463-468
  • 저자
    Kang-Hyun Kim, Yong-Seung Lee, Ha-Na Gu, Boo-Keun Yang, Hee-Tae Cheong, Choon-Keun Park
  • 언어
    영어(ENG)
  • URL
    https://www.earticle.net/Article/A163582

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원문정보

초록

영어
This study investigated the changes of plasminogen activators (PAs) activity, expression and localization of tissue plasminogen activator (tPA) and urokinase plasminogen activator (uPA) during the estrous cycle in pigs. Estrous cycle was sorted into three group by pre-ovulation (Pre-Ov), post-ovulation (Post-Ov) and early to mid-luteal stages (Early to mid-L). Analysis for immunohistochemistry was confirmed by location of tPA and uPA. Porcine uterus tissue was cut into 1 × 1 cm squares, and were incubated in DMEM/F-12 medium for 1 h at 38℃, 5% CO2 for measurement of PA activity. Western blotting was implemented for measurement of PA quantity. In results, the blood vessels and secretory glands were increased in Post-Ov stage than Pre-Ov and Early to mid-L stages. The tPA and uPA was located mainly within lumen of blood vessels and secretory glands. The PA activity in Post-Ov (0.99±0.03) stage were significantly (p<0.01) higher than Pre-Ov stage (0.51±0.03) and Early to mid-L stage (0.21±0.04). Expression of PAs were significantly (p<0.05) higher in Early to mid-L stage than other stages. These results indicate that PAs activity and expression may change in uterus tissue during the estrous cycle in pigs.

목차

ABSTRACT
 INTRODUCTION
 MATERIALS AND METHODS
  Collection and Criterion of Uterus by Estrous Cycle
  PA Activity Assay
  Immunohistochemistry Method
  Western Blot
  Statistical Analysis
 RESULTS
 DISCUSSION
 REFERENCES

키워드

Plasminogen activators Uterus tissue Estrous cycle Pigs

저자

  • Kang-Hyun Kim [ College of Animal Life Sciences, Kangwon National University ]
  • Yong-Seung Lee [ College of Animal Life Sciences, Kangwon National University ]
  • Ha-Na Gu [ College of Animal Life Sciences, Kangwon National University ]
  • Boo-Keun Yang [ College of Animal Life Sciences, Kangwon National University ]
  • Hee-Tae Cheong [ College of Veterinary Medicine, Kangwon National University ]
  • Choon-Keun Park [ College of Animal Life Sciences, Kangwon National University ] Corresponding author

참고문헌

자료제공 : 네이버학술정보

간행물 정보

발행기관

  • 발행기관명
    한국동물생명공학회(구 한국동물번식학회) [The Korean Society of Animal Reproduction and Biotechnology]
  • 설립연도
    1976
  • 분야
    농수해양>축산학
  • 소개
    동물번식생리학, 동물생명공학, 수의학, 인공수정 및 수정란이식을 이용한 동물개량에 관한 이론과 기술의 발전을 통해 학계, 연구계, 산업계 및 양축가 상호간의 협력을 도모함으로써 동물과학발전 및 사회일반의 이익에 기여 한다는 목적을 위해 노력해 나가겠습니다.

간행물

  • 간행물명
    Reproductive & developmental biology
  • 간기
    계간
  • pISSN
    1738-2432
  • eISSN
    2288-0151
  • 수록기간
    1977~2018
  • 십진분류
    KDC 527 DDC 636

이 권호 내 다른 논문 / Reproductive & developmental biology Volume 35 No 4

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